Tandemly repeated sequences in the mitochondrial DNA control region and phylogeography of the pike-perches Stizostedion
Tandemly repeated sequences in the mitochondrial DNA control region and phylogeography of the pike-perches Stizostedion
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DOI：
10.1006/mpev.1998.0530
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发表时间：
1998-12-01
影响因子：
4.1
通讯作者：
Stepien, CA
中科院分区：
文献类型：
--
作者：
Faber, JE;Stepien, CA
线粒体 DNA 控制区的 DNA 序列用于测试梭鲈、Stizostedion（Teleostei：鲈科）之间的系统发育关系，并检查变异模式。序列揭示了两种类型的变异性：单核苷酸多态性和10-至11-碱基对串联重复序列的6至14个拷贝。人们发现串联重复的拷贝数进化得太快，以至于无法在任何分类水平上检测系统发育信号，甚至在种群中也是如此。 Stizostedion 和其他鲈鱼之间串联重复的序列相似性表明一致的进化过程。串联重复的预测折叠及其与终止相关序列的接近表明二级结构介导 d 环、重链和轻链之间的滑链错配。序列的邻接和最大简约分析表明该属在北美和欧亚大陆上分为分支。用分歧时间校准遗传距离支持了这样的假设：Stizostedion 在距今大约 400 万年前，即上新世初期附近，通过北太平洋白令陆桥从欧亚大陆扩散到北美。北美的 S. lucioperca 和 S. volgensis 相距约 275 万年，欧亚的 S. lucioperca 和 S. volgensis 相距约 180 万年，这表明物种形成发生在上新世晚期。 (C) 学术出版社。
DNA sequences from the mitochondrial DNA control region are used to test the phylogeographic relationships among the pike-perches, Stizostedion (Teleostei: Percidae) and to examine patterns of variation. Sequences reveal two types of variability: single nucleotide polymorphisms and 6 to 14 copies of 10- to Il-basepair tandemly repeated sequences. Numbers of copies of the tandem repeats are found to evolve too rapidly to detect phylogenetic signal at any taxonomic level, even among populations. Sequence similarities of the tandem repeats among Stizostedion and other percids suggest concerted evolutionary processes. Predicted folding of the tandem repeats and their proximity to termination-associated sequences indicate that secondary structure mediates slipped-strand mispairing among the d-loop, heavy, and light strands. Neighbor-joining and maximum parsimony analyses of sequences indicate that the genus is divided into clades on the continents of North America and Eurasia. Calibrating genetic distances with divergence times supports the hypothesis that Stizostedion dispersed from Eurasia to North America across a North Pacific Beringial land bridge approximately 4 million years before present, near the beginning of the Pliocene Epoch. The North American S, vitreum and S. canadense appear separated by about 2.75 million years, and the Eurasian S. lucioperca and S. volgensis are diverged by about 1.8 million years, suggesting that speciation occurred during the late Pliocene Epoch. (C) Press Academic Press.