Thymosin β4 enhances repair by organizing connective tissue and preventing the appearance of myofibroblasts
Thymosin β4 enhances repair by organizing connective tissue and preventing the appearance of myofibroblasts
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DOI：
10.1111/j.1749-6632.2010.05483.x
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Hazard, Sprague W., III
中科院分区：
文献类型：
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作者：
Ehrlich, H. Paul;Hazard, Sprague W., III
用胸腺素β 4（T β 4）局部治疗的大鼠切口伤口愈合，瘢痕最小，伤口断裂强度无损失。治疗后的伤口宽度明显更窄。偏振光显微镜检查处理的伤口具有上级组织化胶原纤维，显示红色双折射，这与成熟结缔组织一致。对照切口具有随机组织的胶原纤维，显示与未成熟结缔组织一致的绿色双折射。免疫组织学处理的伤口具有很少的肌成纤维细胞和具有平滑肌肌动蛋白（SMA）染色的应力纤维的成纤维细胞。放置在皮下袋中的聚乙烯醇海绵植入物在第2、3和4天接受载体或100 μ g T β 4。在第14天，经处理的植入物通过偏振光显微镜显示具有强烈黄红双折射的更长、更厚的胶原纤维束。在对照组中，细而薄的胶原纤维束以随机阵列排列，主要是绿色双折射。对照组主要含有肌成纤维细胞，而在T β 4处理的植入物中出现很少的肌成纤维细胞。电子显微镜证实了细胞类型和胶原纤维束组织化的程度。我们的研究结果表明，T β 4治疗的伤口似乎成熟更早，愈合疤痕最小。
Incisional wounds in rats treated locally with thymosin beta 4 (T beta 4) healed with minimal scaring and without loss in wound breaking strength. Treated wounds were significantly narrower in width. Polarized light microscopy treated wounds had superior organized collagen fibers, displaying a red birefringence, which is consistent with mature connective tissue. Control incisions had randomly organized collagen fibers, displaying green birefringence that is consistent with immature connective tissue. Immunohistology treated wounds had few myofibroblasts and fibroblasts with a smooth muscle actin (SMA) stained stress fibers. Polyvinyl alcohol sponge implants placed in subcutaneous pockets received either carrier or 100 mu g of T beta 4 on days 2, 3, and 4. On day 14, treated implants revealed longer, thicker collagen fiber bundles with intense yellow-red birefringence by polarized light microscopy. In controls, fine, thin collagen fiber bundles were arranged in random arrays with predominantly green birefringence. Controls contained mostly myofibroblasts, while few myofibroblasts appeared in T beta 4 treated implants. Electron microscopy confirmed both cell types and the degree of collagen fiber bundle organization. Our results demonstrate that T beta 4 treated wounds appear to mature earlier and heal with minimal scaring.