Production of extracellular enzymes by different isolates of Pochonia chlamydosporia
Production of extracellular enzymes by different isolates of Pochonia chlamydosporia
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DOI：
10.1016/j.mycres.2009.04.005
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发表时间：
2009-08-01
影响因子：
--
通讯作者：
Kerry, Brian
中科院分区：
文献类型：
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作者：
Esteves, Ivania;Peteira, Belkis;Kerry, Brian
首次定量比较了不同菌株的几丁质酶、酯酶、脂肪酶和丝氨酸蛋白酶（VCP1）的活性。分离株在基本液体培养基或补充有1%几丁质、0.2%明胶或2%橄榄油的培养基中生长不同的时间段。使用显色对硝基苯基底物（用于几丁质酶、脂肪酶和酯酶）和对硝基苯胺底物（用于测量蛋白酶VCP 1的活性）在过滤的培养物上清液中定量酶比活性。此外，收集了寄生生长（线虫卵寄生）和腐生性生长（植物根际定殖）的信息。结果表明，厚垣假单胞菌胞外酶的产生受培养基类型的影响（p < 0.05）。酶活性随时间变化（p < 0.05），菌株间差异极显著（p < 0.001），产酶量差异极显著（p < 0.001）。然而，没有显着的关系，发现酶活性和寄生或腐养生长使用肯德尔的协调系数或斯皮尔曼等级相关系数。这些结果提供了有关厚垣假单胞菌产酶的新信息，并表明调节该真菌营养转换的机制是复杂的，取决于多种因素。(C)2009年英国真菌学会。由爱思唯尔有限公司出版。保留所有权利。
For the first time, the specific activities of chitinases, esterases, lipases and a serine protease (VCP1) produced by different isolates of the nematophagous fungus Pochonia chlamydosporia were quantified and compared. The isolates were grown for different time periods in a minimal liquid medium or media supplemented with 1 % chitin, 0.2 % gelatin or 2 % olive oil. Enzyme-specific activities were quantified in filtered culture supernatants using chromogenic p-nitrophenyl substrates (for chitinases, lipases and esterases) and a p-nitroanilide substrate (to measure the activity of the proteinase VCP1). Additionally, information on parasitic growth (nematode egg parasitism) and saprotrophic growth (plant rhizosphere colonisation) was collected. Results showed that the production of extracellular enzymes was influenced by the type of medium (p < 0.05) in which P. chlamydosporia was grown. Enzyme activity differed with time (p < 0.05), and significant differences were found between isolates (p < 0.001) and the amounts of enzymes produced (p < 0.001). However, no significant relationships were found between enzyme activities and parasitic or saprotrophic growth using Kendall's coefficient of concordance or Spearman rank correlation coefficient. The results provided new information about enzyme production in P. chlamydosporia and suggested that the mechanisms which regulate the trophic switch in this fungus are complex and dependent on several factors. (C) 2009 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.