Retrovirus-mediated transfer and expression of the interleukin-3 gene in mouse hematopoietic cells result in a myeloproliferative disorder.
Retrovirus-mediated transfer and expression of the interleukin-3 gene in mouse hematopoietic cells result in a myeloproliferative disorder.
复制标题
逆转录病毒介导的白细胞介素 3 基因在小鼠造血细胞中的转移和表达会导致骨髓增殖性疾病。
DOI：
10.1128/mcb.9.2.798-808.1989
复制
发表时间：
1989
影响因子：
5.3
通讯作者：
Nienhuis,AW
中科院分区：
文献类型：
--
作者：
Wong,PM;Chung,SW;Dunbar,CE;Bodine,DM;Ruscetti,S;Nienhuis,AW
一个高滴度，重组逆转录病毒载体中产生的包装细胞已被用来引入小鼠白细胞介素-3（IL-3）基因到小鼠造血细胞。在脾病灶中观察到IL-3基因的整合和表达，从中可以衍生出不依赖于因子的、持续增殖的细胞系。受感染造血细胞的辐射或遗传性贫血的W/W受体发展为骨髓增殖综合征，其特征为白细胞计数显著升高、骨髓增生和肝、脾肿大。该综合征反映了一个或多个干细胞克隆的增殖，其后代能够重新繁殖第二受体。一只动物主要通过旁分泌机制发展出该综合征。内源性IL-3的产生引起造血细胞的扩增，但似乎并没有改变这些细胞的成熟或自我更新的潜力。
A high-titer, recombinant retroviral vector produced in ψ2 packaging cells has been used to introduce the murine interleukin-3 (IL-3) gene into mouse hematopoietic cells. Integration and expression of the IL-3 gene was observed in spleen foci from which could be derived factor-independent, continuously proliferating cell lines. Irradiated or genetically anemicW/Wvrecipients of infected hematopoietic cells developed a myeloproliferative syndrome characterized by a marked elevation in leukocyte count, bone marrow hyperplasia, and enlargement of the liver and spleen. The syndrome reflected proliferation of one or more stem cell clones, the progeny of which were capable of repopulating secondary recipients. One animal developed the syndrome primarily by a paracrine mechanism. Endogenous IL-3 production caused amplification of hematopoietic cells but did not appear to alter the maturational or self-renewal potential of these cells.