Salmonella SdiA recognizes N-acyl homoserine lactone signals from Pectobacterium carotovorum in vitro, but not in a bacterial soft rot.
Salmonella SdiA recognizes N-acyl homoserine lactone signals from Pectobacterium carotovorum in vitro, but not in a bacterial soft rot.
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沙门氏菌 SdiA 在体外可识别来自胡萝卜果杆菌的 N-酰基高丝氨酸内酯信号，但不能识别细菌软腐病中的 N-酰基高丝氨酸内酯信号。
DOI：
10.1094/mpmi-23-3-0273
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Teplitski,M
中科院分区：
文献类型：
--
作者：
Noel,JT;Joy,J;Smith,JN;Fatica,M;Schneider,KR;Ahmer,BMM;Teplitski,M
Genomes ofSalmonella entericaisolates, including those linked to outbreaks of produce-associated gastroenteritis, containsdiA, which encodes a receptor ofN-acyl homoserine lactones (AHL). AHL are the quorum-sensing signals used by bacteria to coordinately regulate gene expression within -their populations. BecauseS. entericadoes not produce its own AHL, SdiA is hypothesized to function in the interspecies cross-talk with AHL-producing bacteria. Under laboratory conditions,S. entericaresponded to AHL from phytobacteria by upregulating expression ofsrgE. AHL-dependent expression ofsrgErequired a functionalsdiA. Essentially, nosdiA-dependent resolution of thesrgErecombinase-based (RIVET) reporter was observed inside a soft rot formed on a tomato by an AHL-producing strain ofPectobacterium carotovorum. The results of the control experiments suggest thatsdiAis not expressed inside tomato, pepper, green onion, or carrot affected by the soft rot, and the lack ofsdiAexpression in planta preventsSalmonellaspp. from responding to AHL. Despite its inability to detect and respond to AHL during colonization of soft rots,S. entericareached higher final cell numbers inside a tomato soft rot compared with its growth in intact tomato fruit. The synergistic effect was the strongest under the conditions that are typical for the Florida fall/winter production season.
