ATRIAL NATRIURETIC PEPTIDE INHIBITS A CATION CHANNEL IN RENAL INNER MEDULLARY COLLECTING DUCT CELLS
ATRIAL NATRIURETIC PEPTIDE INHIBITS A CATION CHANNEL IN RENAL INNER MEDULLARY COLLECTING DUCT CELLS
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DOI：
10.1126/science.2463673
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发表时间：
1989-01-20
期刊：
影响因子：
56.9
通讯作者：
STANTON, BA
中科院分区：
文献类型：
--
作者：
LIGHT, DB;SCHWIEBERT, EM;STANTON, BA
应用膜片钳技术研究了心房利钠肽（ANP）及其第二信使鸟苷3′，5′-单磷酸鸟苷（cGMP）对肾内髓集管细胞顶膜上氨苄胺敏感阳离子通道的影响。ANP （10-11 M）和二丁基鸟苷3′，5′-单磷酸二丁基鸟苷（10-4M）抑制细胞贴附斑块中的通道，cGMP （10-5M）抑制内向外斑块中的通道。髓内集管是ANP通过其第二信使cGMP调控单离子通道的第一个组织。结果表明，ANP的利钠作用部分与cgmp介导的髓内集管对电致Na+吸收的抑制有关。
The patch-clamp technique was used to examine the effects of atrial natriuretic peptide (ANP) and its second messenger guanosine 3'',5''-monophosphate (cGMP) on an amiloride-sensitive cation channel in the apical membrane of renal inner medullary collecting duct cells. Both ANP (10-11 M) and dibutyryl guanosine 3'',5''-monophosphate (10-4M) inhibited the channel in cell-attached patches, and cGMP (10-5M) inhibited the channel in inside-out patches. The inner medullary collecting duct is the first tissue in which ANP, via its second messenger cGMP, has been shown to regulate single ion channels. The results suggest that the natriuretic action of ANP is related in part to cGMP-mediated inhibition of electrogenic Na+ absorption by the inner medullary collecting duct.