Genomic structure and multiple alternative transcripts of the mouse TRAD/RAD51L3/RAD51D gene, a member of the recA/RAD51 gene family
Genomic structure and multiple alternative transcripts of the mouse TRAD/RAD51L3/RAD51D gene, a member of the recA/RAD51 gene family
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DOI：
10.1016/j.bbaexp.2004.05.006
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发表时间：
2004-08-12
期刊：
影响因子：
--
通讯作者：
Kawabata, T
中科院分区：
文献类型：
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作者：
Kawabata, M;Akiyama, K;Kawabata, T
RecA/RAD 51家族在DNA重组修复中起着重要作用。小鼠RAD 51 L3/TRAD的靶向破坏在胚胎发生期间是致命的，这表明该蛋白质对于发育是必需的。最近，我们报道了多种人RAD 51 L3/TRAD转录本的选择性剪接变体。在这项研究中，我们已经确定了多种小鼠转录本变异。基因组和cDNA克隆的全序列分析证实，外显子-内含子结构服从GT/AG剪接规则，转录本的多样性是由于选择性剪接。此外，我们还通过cDNA 5 ′-末端快速扩增（5 '-RACE）确定了转录起始位点。这些结果表明，小鼠的基因结构与人类非常相似。(C)2004 Elsevier B.V保留所有权利。
The RecA/RAD51 family plays a central role in DNA recombinational repair. The targeted disruption of mouse RAD51L3/TRAD is lethal during embryogenesis, suggesting that this protein is essential for development. Recently, we reported multiple alternative splice variants of human RAD51L3/TRAD transcripts. In this study, we have identified multiple mouse transcript variants. Complete sequence analysis of the genomic and cDNA clones has confirmed that the exon-intron structures obey the GT/AG splicing rule, and that the multiplicity of the transcripts is due to alternative splicing. In addition, we have determined the transcription initiation site by rapid amplification of cDNA 5'-end (5'-RACE). These results show that the mouse gene structure is very similar to that of humans. (C) 2004 Elsevier B.V All rights reserved.