Genome-Wide Identification, Functional Analysis and Expression Profiling of the Aux/IAA Gene Family in Tomato
Genome-Wide Identification, Functional Analysis and Expression Profiling of the Aux/IAA Gene Family in Tomato
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DOI：
10.1093/pcp/pcs022
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发表时间：
2012-04-01
影响因子：
4.9
通讯作者：
Bouzayen, Mondher
中科院分区：
文献类型：
--
作者：
Audran-Delalande, Corinne;Bassa, Carole;Bouzayen, Mondher
生长素是一种中枢激素，对植物生长包括根、芽、花和果实的发育发挥多效性作用。植物激素生长素的感知和信号转导依赖于多个组分的协同作用，其中生长素/吲哚乙酸（Aux/IAA）蛋白起着关键作用。在这项研究中，我们确定和全面分析了整个Aux/IAA基因家族的番茄（茄科植物的参考物种，肉质果实发育的模式植物）。使用专用的单细胞系统的功能表征表明，番茄Aux/IAA蛋白作为生长素依赖性基因转录的活性阻遏物，然而，不同的Aux/IAA成员显示不同水平的阻遏。系统发育分析表明，与拟南芥相比，番茄中的Aux/IAA基因家族略有收缩，非典型蛋白的代表性较低。S1-IAA基因在番茄不同器官和组织中表现出不同的表达模式，其中一些基因对生长素和乙烯的反应也不同，表明Aux/IAAs可能在连接这两条激素信号通路中发挥作用。这里提出的数据揭示了更多的光S1-IAA基因，并提供了新的线索，阐明其在植物发育过程中的功能，并在介导激素串扰。
Auxin is a central hormone that exerts pleiotropic effects on plant growth including the development of roots, shoots, flowers and fruit. The perception and signaling of the plant hormone auxin rely on the cooperative action of several components, among which auxin/indole-3-acetic acid (Aux/IAA) proteins play a pivotal role. In this study, we identified and comprehensively analyzed the entire Aux/IAA gene family in tomato (Solanum lycopersicum), a reference species for Solanaceae plants, and the model plant for fleshy fruit development. Functional characterization using a dedicated single cell system revealed that tomato Aux/IAA proteins function as active repressors of auxin-dependent gene transcription, with, however, different Aux/IAA members displaying varying levels of repression. Phylogenetic analysis indicated that the Aux/IAA gene family is slightly contracted in tomato compared with Arabidopsis, with a lower representation of non-canonical proteins. Sl-IAA genes display distinctive expression pattern in different tomato organs and tissues, and some of them display differential responses to auxin and ethylene, suggesting that Aux/IAAs may play a role in linking both hormone signaling pathways. The data presented here shed more light on Sl-IAA genes and provides new leads towards the elucidation of their function during plant development and in mediating hormone cross-talk.