Compact multi-enzyme pathways in P. pastoris
Compact multi-enzyme pathways in P. pastoris
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DOI：
10.1039/c4cc08502g
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发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Glieder, Anton
中科院分区：
文献类型：
--
作者：
Geier, Martina;Fauland, Pia;Glieder, Anton
我们首次报告了从一个单一的2A肽为基础的多顺反子表达构建体的9个基因的功能性同时表达。通过共表达紫色素和类胡萝卜素生物合成途径，证明了生物合成途径平衡化学生产的可行性和出现的机会。
We report for the first time the functional simultaneous expression of nine genes from a single 2A peptide based polycistronic expression construct. The feasibility and arising opportunities for the biosynthetic pathway balancing for chemical production were demonstrated by the co-expression of the violacein and carotenoid biosynthesis pathways.