Experimental infection and transmission of Leishmania by Lutzomyia cruzi (Diptera: Psychodidae): Aspects of the ecology of parasite-vector interactions
Experimental infection and transmission of Leishmania by Lutzomyia cruzi (Diptera: Psychodidae): Aspects of the ecology of parasite-vector interactions
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DOI：
10.1371/journal.pntd.0005401
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发表时间：
2017-02-01
影响因子：
3.8
通讯作者：
Bianchi Galati, Eunice Aparecida
中科院分区：
文献类型：
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作者：
de Oliveira, Everton Falcao;Oshiro, Elisa Teruya;Bianchi Galati, Eunice Aparecida
在将利什曼原虫传播的媒介归罪之前，应该解决几个参数。这些因素可能包括它被在人类中发现的相同利什曼原虫感染的能力，对水库和人类的吸引力程度，以及在实验室条件下维持寄生虫感染的能力。这项研究评估了克鲁兹丝虫对婴儿利什曼原虫的媒介能力，并收集了有关其庇护亚马逊利什曼原虫能力的信息。实验室饲养的卢。通过给自然感染婴儿钩端螺旋体的狗和感染亚马逊钩端螺旋体的仓鼠喂食，实验感染了克鲁兹。沙蝇对狗和人类的吸引力是通过野生捕获的昆虫来确定的。预计感染鲁氏杆菌患者的日常生存时间。此外，还对这两种寄生虫的生殖器营养周期持续时间和外在潜伏期进行了调查。对两种利什曼原虫的媒介能力进行了调查。捕食寄主的雌性沙蝇平均比例为0.40。对于L.infantum和L.amazonensis，Lu.克鲁兹的实验感染率分别为10.55%和41.56%。两种利什曼原虫的外在潜伏期均为3天，与寄主无关。感染婴儿钩端螺旋体和亚马逊钩端螺旋体的雌性在完成性腺营养周期后的存活预期分别为1.32和0.43。婴儿钩端螺旋体感染与沙蝇寿命之间没有关联，但感染亚马逊钩端螺旋体的苍蝇存活概率明显更高。此外，产卵显著不利于生存。陆川。研究发现，克鲁兹对狗和人都有很强的吸引力。在实验感染的宿主上吃了一顿血后，这两种寄生虫都能够在Lu存活并发展为晚期感染。克鲁兹。然而，仅在感染了亚马孙乳杆菌的沙蝇中证实了传播。总而言之，陆川。CRUZI满足了婴儿乳杆菌传播对载体容量的几个要求。此外，对日本血吸虫也有一定的允许性。
Several parameters should be addressed before incriminating a vector for Leishmania transmission. Those may include its ability to become infected by the same Leishmania species found in humans, the degree of attractiveness for reservoirs and humans and capacity to sustain parasite infection under laboratory conditions. This study evaluated the vectorial capacity of Lutzomyia cruzi for Leishmania infantum and gathered information on its ability to harbor L. amazonensis. Laboratory-reared Lu. cruzi were infected experimentally by feeding them on dogs infected naturally with L. infantum and hamsters infected with L. amazonensis. Sand fly attractiveness to dogs and humans was determined using wild caught insects. The expected daily survival of infected Lu. cruzi, the duration of the gonotrophic cycle, and the extrinsic incubation period were also investigated for both parasites. Vector competence was investigated for both Leishmania species. The mean proportion of female sand flies that fed on hosts was 0.40. For L. infantum and L. amazonensis, Lu. cruzi had experimental infection rates of 10.55% and 41.56%, respectively. The extrinsic incubation period was 3 days for both Leishmania species, regardless of the host. Survival expectancy of females infected with L. infantum and L. amazonensis after completing the gonotrophic cycle was 1.32 and 0.43, respectively. There was no association between L. infantum infection and sand fly longevity, but L. amazonensis-infected flies had significantly greater survival probabilities. Furthermore, egg-laying was significantly detrimental to survival. Lu. cruzi was found to be highly attracted to both dogs and humans. After a bloodmeal on experimentally infected hosts, both parasites were able to survive and develop late-stage infections in Lu. cruzi. However, transmission was demonstrated only for L. amazonensis-infected sand flies. In conclusion, Lu. cruzi fulfilled several of the requirements of vectorial capacity for L. infantum transmission. Moreover, it was also permissive to L. amazonensis.