Palatinose and oleic acid act together to prevent pancreatic islet disruption in nondiabetic obese Zucker rats
Palatinose and oleic acid act together to prevent pancreatic islet disruption in nondiabetic obese Zucker rats
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DOI：
10.2152/jmi.55.183
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发表时间：
2008-08-01
影响因子：
0.7
通讯作者：
Takeda, Eiji
中科院分区：
文献类型：
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作者：
Sato, Kazusa;Arai, Hidekazu;Takeda, Eiji
我们先前表明，8周的饮食含有帕拉金糖（P，一种缓慢吸收的蔗糖类似物）和油酸（O）改善，但饮食含有蔗糖（S）和亚油酸（L）加重Zucker脂肪（fa/fa）大鼠的代谢异常。在这项研究中，我们的目的是确定早期的变化，在大鼠代谢诱导的某些组合的碳水化合物和脂肪酸。具体而言，雄性Zucker肥胖大鼠喂食含有各种碳水化合物（P; S）和脂肪酸（O; L）组合的等热量饮食。4周后，体重，内脏脂肪量，血浆参数（葡萄糖，胰岛素，脂质和脂肪因子），肝脏肥胖和基因表达，脂肪炎症之间没有显着差异，观察饮食组。相比之下，帕拉金糖喂养（PO和PL）大鼠的胰岛较小，纤维化程度低于蔗糖喂养（SO和SL）大鼠。在油酸喂养（PO和SO）大鼠中未观察到亚油酸喂养大鼠胰岛常见的异常α细胞分布和活性caspase-3的散在染色。因此，在SL大鼠中观察到进行性β细胞损失，但在PO大鼠中未观察到。这些发现表明，胰岛可能是将饮食中碳水化合物和脂肪的不同组合的影响转化为代谢变化的初始位点。J. Med. Invest. 55：183 - 195，2008年8月
We showed previously that 8-wk consumption of a diet containing palatinose (P, a slowly-absorbed sucrose analogue) and oleic acid (O) ameliorates but a diet containing sucrose (S) and linoleic acid (L) aggravates metabolic abnormalities in Zucker fatty (fa/ fa) rats. In this study, we aimed to identify early changes inmetabolism in rats induced by certain combinations of carbohydrates and fatty acids. Specifically, male Zucker fatty rats were fed an isocaloric diet containing various combinations of carbohydrates (P; S) and fatty acids (O; L). After 4 wk, no significant differences in bodyweight, visceral fat mass, plasma parameters (glucose, insulin, lipids, and adipokines), hepatic adiposity and gene expression, and adipose inflammation were observed between dietary groups. In contrast, pancreatic islets of palatinose-fed (PO and PL) rats were smaller and less fibrotic than sucrose-fed (SO and SL) rats. The abnormal alpha-cell distribution and sporadic staining of active caspase-3 common to islets of linoleic-acid-fed rats were not observed in oleic-acid-fed (PO and SO) rats. Accordingly, progressive beta-cell loss was seen in SL rats, but not in PO rats. These findings suggest that pancreatic islets may be initial sites that translate the effects of different combinations of dietary carbohydrates and fats intometabolic changes. J. Med. Invest. 55 : 183-195, August, 2008