Breaking Pseudo-Symmetry in Multiantennary Complex N-Glycans Using Lanthanide-Binding Tags and NMR Pseudo-Contact Shifts
Breaking Pseudo-Symmetry in Multiantennary Complex N-Glycans Using Lanthanide-Binding Tags and NMR Pseudo-Contact Shifts
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DOI：
10.1002/anie.201307845
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发表时间：
2013-12-16
影响因子：
16.6
通讯作者：
Jimenez-Barbero, Jesus
中科院分区：
文献类型：
--
作者：
Canales, Angeles;Mallagaray, Alvaro;Jimenez-Barbero, Jesus
分子识别对生命具有重要意义。了解细胞受体与其特定配体之间相互作用的化学基础不仅为疾病中发生的结构和变化提供了功能意义，而且有助于设计创新的治疗方法。在生物编码和将信号转化为细胞效应方面，聚糖由于其无与伦比的编码能力和广泛存在的受体（凝集素）来读取编码的信息而获得了特殊的地位。[1]聚糖序列和形状以及聚糖决定簇与其同源凝集素之间的密切相互作用确保了糖编码信息的流动。[2]因此，除了它们的肽支架之外，糖蛋白在它们的聚糖链中携带第二生物信息源，例如，通过凝集素识别N-聚糖来实现。[3]In在这种情况下，我们在此提出了一种新的NMR方法来单独监测N-聚糖的每个臂A和B的行为（方案1），从而提供了它们在溶液中的构象和相互作用特征的全局视角。在结构上，N-聚糖有一个共同的五角体-
Molecular recognition is of vital significance for life. Understanding the chemical basis of these interactions between cellular receptors and their specific ligands not only gives a functional meaning to structures and changes occurring in diseases but also helps devise innovative therapeutic approaches. In terms of biological coding and translating signals into cellular effects, glycans have gained a particular status, owing to their unsurpassed coding capacity and widespread presence of receptors (lectins) to read the encoded information.[1] The glycan sequence and shape and the intimate interplay between a glycan determinant and its cognate lectin ensure the flow of information of sugar coding.[2] Thus, in addition to their peptide scaffold, glycoproteins carry a second source of bioinformation in their glycan chains, realized, for example, by recognition of N-glycans by lectins.[3]In this context, we herein present a novel NMR approach to individually monitor the behavior of each arm, A and B, of N-glycans (Scheme 1) and thereby provide a global perspective of their conformational and interaction features in solution. Structurally, N-glycans have a common pentasac-