A simple method for sexing living Anopheles larvae (Diptera, Culicidae)
A simple method for sexing living Anopheles larvae (Diptera, Culicidae)
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DOI：
10.1093/aesa/50.1.104
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发表时间：
1957-01-01
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影响因子：
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通讯作者：
JONES, JACK COLVARD
中科院分区：
文献类型：
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作者：
JONES, JACK COLVARD
第三阶段四斑按蚊（Anopheles quadramaculatus）幼虫的性别鉴定准确率约为90%。当色素沉着处于中间状态时，无法通过这种方法准确地确定幼虫的性别。四龄幼虫可迅速和准确地（100%）性别的简单显微镜检查的成虫触角盘下生长的幼虫触角。在男性幼虫的光盘总是更大，更充分的分化比女性的相同年龄。他们最容易性别后立即换毛从3d阶段，但该方法是准确的整个第四体育场。最后阶段的幼虫也可以通过检查性腺或形成外生殖器的圆盘来确定性别，准确率约为90%：两者都只在年轻的雄性幼虫中可见。本文简要描述了成虫触角盘的解剖结构，并比较了两性间触角盘的发育阶段。第1、2期幼虫不能用任何方法进行性别鉴定。|| 摘要编辑：作者
Third stage Anopheles quadrimaculatus Say larvae may be sexed with about 90% accuracy heavily pigmented ("black") larvae develop into females, very lightly pigmented larvae are males. Larvae cannot be accurately sexed by this means when the pigmentation is intermediate. Fourth instars may be rapidly and accurately (100%) sexed by simple microscopic examination of the imaginal antennal discs growing just under the larval antennae. In male larvae the discs are always larger and more fully differentiated than in females of the same age. They are most easily sexed immediately after the molt from the 3d stage, but the method is accurate throughout the 4th stadium. Last stage larvae can also be sexed with about 90% accuracy by examination of gonads or of the discs that form the external genitalia: both are visible only in young male larvae. The anatomy of the imaginal antennal discs is briefly described and the developmental stages between the sexes compared. Larvae in the 1st 2 stages could not be sexed by any method used. || ABSTRACT AUTHORS: Author