Acid vacuolar invertase 1 (PbrAc-Inv1) and invertase inhibitor 5 (PbrII5) were involved in sucrose hydrolysis during postharvest pear storage
Acid vacuolar invertase 1 (PbrAc-Inv1) and invertase inhibitor 5 (PbrII5) were involved in sucrose hydrolysis during postharvest pear storage
复制标题
酸性液泡转化酶 1 (PbrAc-Inv1) 和转化酶抑制剂 5 (PbrII5) 参与梨采后贮藏过程中的蔗糖水解
DOI：
10.1016/j.foodchem.2020.126635
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发表时间：
2020-08-01
期刊：
影响因子：
8.8
通讯作者：
Zhang, Shao-ling
中科院分区：
文献类型：
--
作者：
Ma, Min;Wang, Li-bin;Zhang, Shao-ling
在梨果实中，蔗糖主要分布在液泡中，贮藏过程中蔗糖含量的变化与液泡转化酶（VI）活性的变化有关。然而，这种现象背后的分子机制直到最近才被阐明。为此，在本研究中进行了代谢产物、酶活性、转录组、定量实时PCR（qRT-PCR）、生物信息学、亚细胞定位和瞬时过表达测定的组合，以鉴定在'Housur'梨贮藏期间参与蔗糖降解的酸性转化酶1（PbrAc-Inv 1）和转化酶抑制剂5（PbrII 5）。PbrAc-Inv 1和PbrII 5均定位于液泡膜。PbrAc-Inv 1能加速蔗糖降解，而PbrII 5能与PbrAc-Inv 1结合形成失活复合物，下调VI活性，抑制蔗糖降解。基于结构域取代后的生物层干涉（BLI）结果，PbrAc-Inv 1中催化'WEC-P/V-D'框左侧的结构域可能在其与PbrII 5的相互作用中起关键作用。
In pear, sucrose was mainly distributed in vacuole; and the alternation of sucrose abundance was associated the change of vacuolar invertase (VI) activity during fruit storage. However, the molecular mechanism beneath such phenomenon has not been clarified until recently. For this, a combination of metabolite, enzyme activity, transcriptome, quantitative real-time PCR (qRT-PCR), bioinformation, subcellular localization, and transient overexpression assay was conducted in this study to identify the acid invertase 1 (PbrAc-Inv1) and invertase inhibitor 5 (PbrII5) involved in sucrose degradation during 'Housur' pear storage. Both PbrAc-Inv1 and PbrII5 were located in vacuolar membrane. PbrAc-Inv1 could accelerate sucrose degradation; on the other hand, PbrII5 could bind with PbrAc-Inv1 to form a inactive complex, downregulate the VI activity, and suppressed sucrose decomposition. Based on Bio-layer interferometry (BLI) result after domain substitution, the domain on the left of catalytic 'WEC-P/V-D' box in PbrAc-Inv1 might played a key role in its interaction with PbrII5.