Visualization of cytoskeletal elements by the atomic force microscope
Visualization of cytoskeletal elements by the atomic force microscope
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DOI：
10.1016/j.ultramic.2004.09.008
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发表时间：
2005-02-01
期刊：
影响因子：
2.2
通讯作者：
Sokolov, I
中科院分区：
文献类型：
--
作者：
Berdyyeva, T;Woodworth, CD;Sokolov, I
我们描述了原子力显微镜(AFM)在人类包皮上皮细胞中直接显示环状骨骼纤维的新应用。用低浓度的非离子清洗剂Triton X-100去除膜。可溶性蛋白质。细胞中的细胞器。然后，可以在液体或风干的环境条件下直接显示剩余的细胞骨架。这两种类型的扫描提供了补充信息。在液体中扫描可以看到细胞骨架的表面细丝，而在空气中扫描可以显示细胞骨架纤维的表面细丝和总体积。观察到的最小纤维直径约为50 nm。这项技术的横向分辨率约为20 nm，可以通过选择锋利的AFM嘴唇将其提高到单纳米级。由于原子力显微镜是一种真正的3D技术，我们能够根据其密度和体积来量化观察到的细胞骨架。纤维的类型可以通过它们的大小来识别，类似于电子显微镜。(C)2004爱思唯尔B.V.保留所有权利。
We describe a novel application of atomic force microscopy (AFM) to directly visualize cyloskeletal fibers in human foreskin epithelial cells. The nonionic detergent Triton X-100 in a low concentration was used 10 remove the membrane. soluble proteins. and organelles from the cell. The remaining cytoskeleton can then be directly visualized in either liquid or air-dried ambient conditions. These two types of scanning provide complimentary information. Scanning in liquid visualizes the surface filaments of the cytoskeleton, whereas scanning in air shows both the surface filaments and the total "volume" of the cytoskeletal fibers. The smallest fibers observed were ca. 50 nm in diameter. The lateral resolution of this technique was ca.20nm, which can be increased to a single nanometer level by choosing sharp-er AFM lips. Because the AFM is a true 3D technique, we are able to quantify the observed cytoskeleton by its density and volume. The types of fibers call be identified by their size, similar to electron microscopy. (C) 2004 Elsevier B.V. All rights reserved.