Identification of genes associated with head and neck carcinogenesis by cDNA microarray comparison between matched primary normal epithelial and squamous carcinoma cells
Identification of genes associated with head and neck carcinogenesis by cDNA microarray comparison between matched primary normal epithelial and squamous carcinoma cells
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DOI：
10.1038/sj.onc.1205351
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发表时间：
2002-04-18
期刊：
影响因子：
8
通讯作者：
Foulkes, WD
中科院分区：
文献类型：
--
作者：
Al Moustafa, AE;Alaoui-Jamali, MA;Foulkes, WD
为了确定基因参与头颈癌的发生，我们比较了基因表达谱匹配的原代正常上皮细胞和原发性头颈癌细胞从同一患者。由12530个人类基因组成的cDNA微阵列分析揭示了213个基因表达的显著变化，其中91个基因被上调，122个基因被下调。这一完整的基因列表包括与信号转导（生长因子）、细胞结构、细胞周期、转录、凋亡和细胞间粘附相关的基因。进一步分析涉及细胞-细胞相互作用的9个基因，使用蛋白质印迹和/或逆转录（RT）-PCR的4对细胞系支持我们的微阵列分析的可靠性。更具体地说，我们的研究提供了第一个证据，即与正常细胞相比，在头颈部鳞状细胞癌（HNSCC）中，claudin-7和连接蛋白31.1下调。这些发现提供了大量关于与头颈癌发生相关的基因表达谱的信息，也代表了HNSCC预防和/或治疗的潜在靶点的来源。
In order to identify genes involved in head and neck carcinogenesis, we compared the gene expression profile in matched primary normal epithelial cells and primary head and neck cancer cells from the same patients. A cDNA microarray analysis consisting of 12530 human genes revealed significant changes in the expression of 213 genes, with 91 genes being up-regulated and 122 being dowm-regulated. This comprehensive list of genes includes those associated with signal transduction (growth factors), cell structure, cell cycle, transcription, apoptosis, and cell-cell adhesion. Further analysis of nine genes involved in cell-cell interaction, using Western blot and/or reverse transcription (RT)-PCR of four paired cell lines supported the reliability of our microarray analysis. More specifically, our study provides the first evidence that claudin-7 and connexin 31.1 are down-regulated in head and neck squamous cell carcinomas (HNSCC) compared to normal cells. These findings provide a large body of information regarding gene expression profiles associated with head and neck carcinogenesis, and also represent a source of potential targets for HNSCC prevention and/or therapeutics.