Fractionation of hepatic nonparenchymal cells.
Fractionation of hepatic nonparenchymal cells.
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DOI：
10.1100/tsw.2002.283
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发表时间：
2002-05-16
影响因子：
--
通讯作者：
Graham JM
中科院分区：
文献类型：
--
作者：
Graham JM
哺乳动物肝细胞的大部分实质细胞可以通过非常低的速度(50g)离心法去除，但需要一个简单的低密度屏障(1.096 g/ml)才能从含有所有低密度非实质细胞的50g上清液中去除剩余的实质细胞。连续的Nycodenz梯度可以在分析的基础上提供令人满意的Kupffer细胞、星状细胞和内皮细胞的分辨率，但不同类型的细胞的分离在制备上是不够的。然而，通过低密度碘二醇屏障的浮选可以令人满意地浓缩密度最小的非实质细胞--星状细胞。
The majority of parenchymal cells from mammalian liver cells can be removed by very low speed centrifugation (50 g) but a simple low-density barrier (1.096 g/ml) is required to remove the remaining parenchymal cells from the 50-g supernatant which contains all of the lower density nonparenchymal cells. Continuous gradients of Nycodenz can provide satisfactory resolution of Kupffer, stellate, and endothelial cells on an analytical basis but the separation of different cell types is not sufficient preparatively. Flotation through a low-density iodixanol barrier can, however, provide a satisfactory enrichment of the least dense nonparenchymal cell – the stellate cells.