Behavioral responses during the forced swim test are not affected by anti-inflammatory agents or acute illness induced by lipopolysaccharide
Behavioral responses during the forced swim test are not affected by anti-inflammatory agents or acute illness induced by lipopolysaccharide
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DOI：
10.1016/j.bbr.2004.11.024
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发表时间：
2005-05-07
影响因子：
2.7
通讯作者：
Bordner, KA
中科院分区：
文献类型：
--
作者：
Deak, T;Bellamy, C;Bordner, KA
促炎细胞因子和其他传统上与免疫功能相关的分子已被牵连到调节应激源暴露的行为和生理后果。也有证据表明，细胞因子在抑郁人群中异常表达，这表明它们可能在抑郁症/绝望相关过程的发展中发挥病因学作用。因此，我们进行了一系列实验，以确定已知的抑制中枢神经系统细胞因子活性或炎症反应的药物是否会改变强迫游泳测试(FST，一种抑郁/行为绝望的动物模型)中行为反应的正常进展。成年雄性SD大鼠腹腔注射消炎痛(1或10 mg/kg)、α-MSH0.25或0.5μg icv或米诺环素(20或40 mg/kg)。在FST的每一天之前，都要进行行为评估。在测试的任何一天，注射消炎痛、α-MSH或米诺环素对FST期间的不动反应的发展没有影响。在第二个系列的实验中，我们检查了强迫游泳时的行为反应是否会受到单次注射脂多糖(LPS)引起的急性疾病的影响。急性注射脂多糖(10或100μg/kg，ip)无论何时注射，在FST期间对行为反应都没有影响，尽管在相同剂量的内毒素后，社会行为明显减少。从这些研究中，我们得出结论：(A)内源性炎症介质似乎不参与FST期间行为反应的正常进展，以及(B)FST期间的行为反应不受急性全身注射内毒素的影响。©2004爱思唯尔B.V.保留所有权利。
Pro-inflammatory cytokines and other molecules traditionally associated with immune function have been implicated in mediating behavioral and physiological consequences of stressor exposure. There is also evidence that cytokines are aberrantly expressed in depressive populations, suggesting they may play an etiological role in the development of depression/despair-related processes. Thus, we conducted a series of experiments to determine whether agents known to suppress cytokine activity or inflammatory responses in the CNS would alter the normal progression of behavioral responses during the forced swim test (FST, an animal model of depression/behavioral despair). Adult male Sprague-Dawley rats were injected with indomethacin (1 or 10 mg/kg intraperitoneally (i.p.)), α-MSH (0.25 or 0.5 μ g icv), or minocycline (20 or 40mg/kg i.p.) prior to each day of the FST and behavioral assessments were performed. Injection of indomethacin, α-MSH, or minocycline had no effect on the development of the immobility response during the FST on either day of testing. In a second series of experiments, we examined whether behavioral responses during forced swim would be affected by acute illness induced by a single injection of lipopolysaccharide (LPS). Acute injection of LPS (10 or 100 μ g/kg i.p.) had no effect on behavioral responding during the FST irrespective of when it was injected, despite pronounced reductions in social behavior following these same doses of LPS. From these studies, we conclude that (a) endogenous inflammatory mediators do not appear to be involved in the normal progression of behavioral responses during the FST, and (b) behavioral responses during the FST are not affected by acute systemic injection of LPS. © 2004 Elsevier B.V. All rights reserved.