β-Glucuronidase activity during development of the male gametophyte from transgenic and non-transgenic plants
β-Glucuronidase activity during development of the male gametophyte from transgenic and non-transgenic plants
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转基因和非转基因植物雄配子体发育过程中的β-葡萄糖醛酸酶活性
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
R. Bino
中科院分区：
文献类型：
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作者：
L. Plegt;R. Bino
摘要对马铃薯、烟草和番茄花药和花粉中的β -葡糖醛酸酶（GUS）进行了组织化学分析。在含有嵌合GUS基因的转基因植物和未转化植物中测定GUS活性。在转基因植物减数分裂前期和减数分裂孢子发育阶段的花药中，指示GUS活性的内生沉淀在维管柱、连生膜和气孔细胞中一致存在，而在绒毡层和孢子组织中未发现活性。在相似的阶段，未转化植物的花药切片没有显示任何靛蓝染色。在小孢子和花粉发育的后期，转基因和未转化植物的花药在绒毡层和产孢细胞中均表现出高水平的GUS活性。在转基因植物的花药中，GUS也存在于维管柱、连生柄和气孔中。这些结果表明，转基因马铃薯、烟草和番茄花药中嵌合的GUS基因产物具有组织特异性。他们还发现一个内源GUS基因在转化和未转化植物的绒毡层和花粉中以时间和空间特异性的方式表达。
SummaryBeta-glucuronidase (GUS) was histochemically analyzed in anthers and pollen of potato, tobacco and tomato. GUS activity was determined in transgenic plants containing a chimaeric GUS gene and in untransformed plants. In anthers of transgenic plants at premeiotic and meiotic stages of sporogenous development, indigogenic precipitation indicative of GUS activity was consistently manifest in cells of the vascular cylinder, the connectivum and the stomium while no activity was found in the tapetal and sporogenous tissues. At similar stages, anther sections of untransformed plants did not show any indigo blue staining. At later stages of microspore and pollen development, anthers of both transgenic and untransformed plants demonstrated consistently high levels of GUS activity in tapetal and sporogenic cells. In anthers of transgenic plants, GUS was also present in the vascular cylinder, the connectivum and the stomium. These results indicate that in anthers of transgenic potato, tobacco and tomato the chimaeric GUS gene product was localized tissue specifically. They also show that an endogenous GUS gene was expressed in a temporal- and spatial-specific manner in the tapetum and pollen of both transformed and untransformed plants.