Analysis of CUL-5 expression in breast epithelial cells, breast cancer cell lines, normal tissues and tumor tissues.
Analysis of CUL-5 expression in breast epithelial cells, breast cancer cell lines, normal tissues and tumor tissues.
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DOI：
10.1186/1476-4598-2-40
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发表时间：
2003-11-25
期刊：
影响因子：
37.3
通讯作者：
Husain A
中科院分区：
文献类型：
--
作者：
Fay MJ;Longo KA;Karathanasis GA;Shope DM;Mandernach CJ;Leong JR;Hicks A;Pherson K;Husain A
CUL-5（11 q 22-23）的染色体定位与乳腺癌中的洛缺失相关，提示CUL-5可能是一种肿瘤抑制基因。本研究的目的是确定CUL-5在乳腺上皮细胞与乳腺癌细胞系以及正常人体组织与人体肿瘤中是否存在差异表达。用RT-PCR、北方印迹分析和Western印迹分析检测CUL-5在乳腺上皮细胞（HMEC，MCF-10A）和乳腺癌细胞（MCF-7，MDA-MB-231）中的表达。通过RT-PCR评估这些细胞中其他CUL家族成员（CUL-1、CUL-2、CUL-3、CUL-4A和CUL-4 B）的mRNA表达。正常人组织表达阵列和癌症谱阵列分别用于检查正常人组织和匹配的正常组织与肿瘤组织中的CUL-5表达。CUL-5在mRNA和蛋白质水平上由乳腺上皮细胞（HMEC，MCF-10A）和乳腺癌细胞（MCF-7，MDA-MB-231）表达。这些细胞也表达其他CUL家族成员的mRNA。正常人组织表达阵列显示CUL-5广泛表达。癌症分析阵列显示，与匹配的正常组织相比，82%（41/50）的乳腺癌显示CUL-5表达降低。对于50例匹配的乳腺组织，肿瘤组织中CUL-5的表达与正常组织相比在统计学上显著降低~2.2倍（P < 0.0001）。数据表明，与乳腺上皮细胞（HMEC，MCF-10A）相比，乳腺癌细胞系（MCF-7，MDA-MB-231）中的CUL-5表达没有明显降低。与匹配的正常组织相比，乳腺肿瘤组织中CUL-5表达的降低支持了CUL-5表达降低可能在乳腺肿瘤发生中起作用的假设。
The chromosomal location of CUL-5 (11q 22-23) is associated with LOH in breast cancer, suggesting that CUL-5 may be a tumor suppressor. The purpose of this research was to determine if there is differential expression of CUL-5 in breast epithelial cells versus breast cancer cell lines, and normal human tissues versus human tumors. The expression of CUL-5 in breast epithelial cells (HMEC, MCF-10A), and breast cancer cells (MCF-7, MDA-MB-231) was examined using RT-PCR, Northern blot analysis, and Western blot analysis. The expression of mRNA for other CUL family members (CUL-1, -2, -3, -4A, and -4B) in these cells was evaluated by RT-PCR. A normal human tissue expression array and a cancer profiling array were used to examine CUL-5 expression in normal human tissues and matched normal tissues versus tumor tissues, respectively. CUL-5 is expressed at the mRNA and protein levels by breast epithelial cells (HMEC, MCF-10A) and breast cancer cells (MCF-7, MDA-MB-231). These cells also express mRNA for other CUL family members. The normal human tissue expression array revealed that CUL-5 is widely expressed. The cancer profiling array revealed that 82% (41/50) of the breast cancers demonstrated a decrease in CUL-5 expression versus the matched normal tissue. For the 50 cases of matched breast tissue there was a statistically significant ~2.2 fold decreased expression of CUL-5 in tumor tissue versus normal tissue (P < 0.0001). The data demonstrate no apparent decrease in CUL-5 expression in the breast cancer cell lines (MCF-7, MDA-MB-231) versus the breast epithelial cells (HMEC, MCF-10A). The decrease in CUL-5 expression in breast tumor tissue versus matched normal tissue supports the hypothesis that decreased expression of CUL-5 may play a role in breast tumorigenesis.