Characterization a Novel Butyric Acid-Producing Bacterium Collinsella aerofaciens Subsp. Shenzhenensis Subsp. Nov.
Characterization a Novel Butyric Acid-Producing Bacterium Collinsella aerofaciens Subsp. Shenzhenensis Subsp. Nov.
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新型产丁酸细菌 Collinsella aerofaciens Subsp. 的表征。
DOI：
10.3390/microorganisms7030078
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发表时间：
2019-03-13
期刊：
影响因子：
4.5
通讯作者：
Xiao, Liang
中科院分区：
文献类型：
--
作者：
Qin, Panpan;Zou, Yuanqiang;Xiao, Liang
丁酸产生菌可以生物合成丁酸并减轻炎症性疾病。然而，很少有研究报道Collinsella属具有产生丁酸的能力。在这里，我们的研究描述了一种柯林斯菌菌株，这是一种能够产生丁酸的杆状专性厌氧菌。该微生物分离自人肠道，并且发现最佳生长条件为37 °C，在pH 6.5的PYG培养基上。16 S rRNA基因序列分析表明，该菌与C. aerofaciens ATCC 25986 T，高于区分两个种的阈值（98.65%）。DNA-DNA数字杂交和平均核苷酸同源性值也支持该微生物属于C.产气菌TF 06 -26与C.产D-乳糖、D-果糖和D-麦芽糖的菌株在pH 5和0.2%（w/v）胆酸盐条件下均能生长，表明该菌株是一个新的亚种。比较基因组分析表明，丁酸激酶和磷酸丁酰转移酶的编码完全由该菌株，表明一个特定的丁酸生产功能的C。Collinsella属中的产气菌亚种。因此，Collinsella aerofaciens subsp.深圳亚种nov.，以设定菌株TF 06 - 26 T（=CGMCC 1.5216T = DSM 105138 T）为模式菌株。
Butyrate-producing bacteria can biosynthesize butyrate and alleviate inflammatory diseases. However, few studies have reported that the genus Collinsella has the ability to produce butyric acid. Here, our study depicts a Collinsella strain, which is a rod-shaped obligate anaerobe that is able to produce butyric acid. This microorganism was isolated from a human gut, and the optimal growth conditions were found to be 37 °C on PYG medium with pH 6.5. The 16S rRNA gene sequence demonstrated that this microorganism shared 99.93% similarity with C. aerofaciens ATCC 25986T, which was higher than the threshold (98.65%) for differentiating two species. Digital DNA–DNA hybridization and average nucleotide identity values also supported that this microorganism belonged to the species C. aerofaciens. Distinct phenotypic characteristics between TF06-26 and the type strain of C. aerofaciens, such as the fermentation of D-lactose, D-fructose and D-maltose, positive growth under pH 5 and 0.2% (w/v) cholate, suggested this strain was a novel subspecies. Comparative genome analysis revealed that butyric acid kinase and phosphate butyryltransferase enzymes were coded exclusively by this strain, indicating a specific butyric acid-producing function of this C. aerofaciens subspecies within the genus Collinsella. Thus, Collinsella aerofaciens subsp. shenzhenensis subsp. nov. was proposed, with set strain TF06-26T (=CGMCC 1.5216T = DSM 105138T) as the type strain.