Detecting tumor response to treatment using hyperpolarized 13C magnetic resonance imaging and spectroscopy
Detecting tumor response to treatment using hyperpolarized 13C magnetic resonance imaging and spectroscopy
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DOI：
10.1038/nm1650
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发表时间：
2007-11-01
期刊：
影响因子：
82.9
通讯作者：
Brindle, Kevin M.
中科院分区：
文献类型：
--
作者：
Day, Sam E.;Kettunen, Mikko I.;Brindle, Kevin M.
在某些情况下，对治疗的早期肿瘤反应的测量已经显示出可以预测治疗结果。我们表明，在淋巴瘤小鼠静脉注射超极化[1-C-13]丙酮酸盐，乳酸脱氢酶催化的C-13标记之间的羧基丙酮酸盐和乳酸在肿瘤中的流量可以测量使用13 C磁共振光谱和光谱成像，这种流量在化疗24小时内被抑制。药物治疗后测量的通量的减少和肿瘤细胞死亡的诱导可以通过辅酶NAD（H）的损失和肿瘤中乳酸盐和酶浓度的降低来解释。该技术可以为临床评估肿瘤对治疗的反应提供一种新的方法。
Measurements of early tumor responses to therapy have been shown, in some cases, to predict treatment outcome. We show in lymphoma-bearing mice injected intravenously with hyperpolarized [1-C-13] pyruvate that the lactate dehydrogenase-catalyzed flux of C-13 label between the carboxyl groups of pyruvate and lactate in the tumor can be measured using 13C magnetic resonance spectroscopy and spectroscopic imaging, and that this flux is inhibited within 24 h of chemotherapy. The reduction in the measured flux after drug treatment and the induction of tumor cell death can be explained by loss of the coenzyme NAD(H) and decreases in concentrations of lactate and enzyme in the tumors. The technique could provide a new way to assess tumor responses to treatment in the clinic.