Data in support of transcriptional regulation and function of Fas-antisense long noncoding RNA during human erythropoiesis.
Data in support of transcriptional regulation and function of Fas-antisense long noncoding RNA during human erythropoiesis.
复制标题
支持人类红细胞生成过程中 Fas 反义长非编码 RNA 的转录调控和功能的数据。
DOI：
10.1016/j.dib.2016.03.106
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发表时间：
2016
期刊：
影响因子：
1.2
通讯作者：
Wilber,Andrew
中科院分区：
文献类型：
--
作者：
Villamizar,Olga;Chambers,ChristopherB;Mo,Yin-Yuan;Torry,DonaldS;Hofstrand,Reese;Riberdy,JaniceM;Persons,DerekA;Wilber,Andrew
本文描述了与题为“Fas反义长非编码RNA在人红细胞成熟过程中差异表达并赋予对Fas介导的细胞死亡的抵抗力”的研究文章相关的数据[1]。长链非编码RNA（lncRNA）因其调节基因表达的多个步骤的能力而日益受到重视。虽然最近的研究表明，许多lncRNA是功能性的，但它们在整个人类生物学中的作用范围在很大程度上是不确定的，包括人类红细胞发育（红细胞生成）。在这里，我们包括使用商业qPCR阵列在人红细胞生成的早期、中期和晚期期间82种lncRNA的表达数据。根据这些数据，我们鉴定了研究文章中描述的lncRNA Fas反义1（Fas-AS 1或Saf）。还包括lncRNA Saf的5′非翻译序列（UTR），其中鉴定了转录因子靶序列。定量RT-PCR数据显示了K562人红白血病细胞和来源于人CD 34+细胞的成熟成红细胞中关键红细胞转录因子加塔-1和KLF 1的相对水平。使用随机六聚体与oligo（dT）18制备的cDNA的终点和定量RT-PCR数据显示，lncRNA Saf没有被有效地聚腺苷酸化。最后，我们包括流式细胞术直方图证明Fas水平的成熟成红细胞来源于人CD 34+细胞转导使用模拟条件或慢病毒颗粒编码的Saf。
This paper describes data related to a research article titled, “Fas-antisense long noncoding RNA is differentially expressed during maturation of human erythrocytes and confers resistance to Fas-mediated cell death” [1]. Long noncoding RNAs (lncRNAs) are increasingly appreciated for their capacity to regulate many steps of gene expression. While recent studies suggest that many lncRNAs are functional, the scope of their actions throughout human biology is largely undefined including human red blood cell development (erythropoiesis). Here we include expression data for 82 lncRNAs during early, intermediate and late stages of human erythropoiesis using a commercial qPCR Array. From these data, we identified lncRNA Fas-antisense 1 (Fas-AS1 or Saf) described in the research article. Also included are 5′ untranslated sequences (UTR) for lncRNA Saf with transcription factor target sequences identified. Quantitative RT-PCR data demonstrate relative levels of critical erythroid transcription factors, GATA-1 and KLF1, in K562 human erythroleukemia cells and maturing erythroblasts derived from human CD34+cells. End point and quantitative RT-PCR data for cDNA prepared using random hexamers versus oligo(dT)18revealed that lncRNA Saf is not effectively polyadenylated. Finally, we include flow cytometry histograms demonstrating Fas levels on maturing erythroblasts derived from human CD34+cells transduced using mock conditions or with lentivirus particles encoding for Saf.