Comparison of Picrosirius Red Staining With Second Harmonic Generation Imaging for the Quantification of Clinically Relevant Collagen Fiber Features in Histopathology Samples
Comparison of Picrosirius Red Staining With Second Harmonic Generation Imaging for the Quantification of Clinically Relevant Collagen Fiber Features in Histopathology Samples
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DOI：
10.1369/0022155416659249
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发表时间：
2016-09-01
影响因子：
3.2
通讯作者：
Eliceiri, Kevin W.
中科院分区：
文献类型：
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作者：
Drifka, Cole R.;Loeffler, Agnes G.;Eliceiri, Kevin W.
基质胶原排列已被证明在多种癌症和其他伴有纤维化的疾病中具有临床意义。虽然胶原蛋白变化的许多生物学和临床重要性已在实验环境中使用二次谐波发生 (SHG) 成像得到证明，但目前在常规临床病理学实践中的实施尚令人望而却步。为了将胶原蛋白组织的评估转化为常规病理学工作流程，需要检查替代可视化方法。本研究的目的是定量比较 SHG 显微镜和常用的天狼星红染色剂与标准偏振显微镜 (PSR-POL) 生成的胶原蛋白指标。使用人类胰腺癌作为模型，将每种技术与已建立的图像分割和纤维跟踪算法进行定量比较，其特征是具有重组胶原纤维的明显基质。重要的是，PSR-POL 在良性和癌性组织中的大多数胶原蛋白指标上产生了与 SHG 测量的相似的定量趋势。我们发现值得注意的是，与 SHG 成像相比，PSR-POL 检测到更高的纤维数量、排列、长度、直线度和宽度，但仍然与 SHG 结果具有良好的相关性。 PSR-POL 可以在常规临床病理实验室中为某些类型的胶原蛋白定量提供足够的附加信息。
Stromal collagen alignment has been shown to have clinical significance in a variety of cancers and in other diseases accompanied by fibrosis. While much of the biological and clinical importance of collagen changes has been demonstrated using second harmonic generation (SHG) imaging in experimental settings, implementation into routine clinical pathology practice is currently prohibitive. To translate the assessment of collagen organization into routine pathology workflow, a surrogate visualization method needs to be examined. The objective of the present study was to quantitatively compare collagen metrics generated from SHG microscopy and commonly available picrosirius red stain with standard polarization microscopy (PSR-POL). Each technique was quantitatively compared with established image segmentation and fiber tracking algorithms using human pancreatic cancer as a model, which is characterized by a pronounced stroma with reorganized collagen fibers. Importantly, PSR-POL produced similar quantitative trends for most collagen metrics in benign and cancerous tissues as measured by SHG. We found it notable that PSR-POL detects higher fiber counts, alignment, length, straightness, and width compared with SHG imaging but still correlates well with SHG results. PSR-POL may provide sufficient and additional information in a conventional clinical pathology laboratory for certain types of collagen quantification.