Noninvasive measurement of rat intraocular pressure with the Tono-Pen.
Noninvasive measurement of rat intraocular pressure with the Tono-Pen.
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发表时间：
1993-02
影响因子：
4.4
通讯作者：
C. Moore;S. Milne;J. Morrison
中科院分区：
文献类型：
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作者：
C. Moore;S. Milne;J. Morrison
目的评价Tono-Pen 2眼压计在活体大鼠眼内的测量效果。方法：将20只麻醉的成年棕色挪威大鼠（第1组）的一只眼睛插管，同时连接到注射器和带有图表记录器的压力传感器。我们将IOP从15 mmHg增加到45 mmHg，增量为5 mmHg，并使用Tono-Pen 2眼压计在每个压力增量下获得15个连续读数（忽略仪器生成的平均值）。为了测试tonopen测量未知IOP的能力，在另外20只动物（第2组）中随机改变传感器压力，并以掩蔽方式获得tonopen读数。结果每只动物的平均tonopen读数与传感器眼压之间的回归方程为y = 4.54 + 0.79x（r = 0.98）。第2组平均tonopen值与传感器IOP作图，得出回归公式y = 4.75 + 0.78x（r = 0.94）。方法比较分析表明，tonopen显著高估了低IOP（或= 30 mmHg）时的压力。使用双因素方差分析，确定第2组数据与第1组数据无显著差异（P >或= 0.76）。由于这种一致性，我们生成了Tono-Pen读数的95%预测区间的校正因子。结论Tono-Pen 2可用于正常大鼠眼眼压的测量。
PURPOSE The purpose of this study was to evaluate the Tono-Pen 2 tonometer for measuring intraocular pressure (IOP) in the living rat eye. METHODS One eye from each of 20 adult, anesthetized brown Norway rats (group 1) was cannulated and simultaneously connected to a syringe and a pressure transducer with a chart recorder. We increased IOP from 15 to 45 mmHg in 5-mmHg increments and obtained 15 consecutive readings (ignoring instrument-generated averages) at each pressure increment with a Tono-Pen 2 tonometer. To test the tonopen's ability to measure unknown IOP, transducer pressures were varied randomly in 20 additional animals (group 2), and tonopen readings were obtained in masked fashion. RESULTS Plotting the mean tonopen readings for each animal against transducer IOP produced a regression formula of y = 4.54 + 0.79x (r = 0.98). Mean group 2 tonopen values plotted against transducer IOP yielded a regression formula of y = 4.75 + 0.78x (r = 0.94). A method comparison analysis showed that the tonopen significantly overestimates pressures at low IOP ( or = 30 mmHg). Using two-way analysis of variance, it was determined that the group 2 data did not differ significantly from the group 1 data (P > or = 0.76). Because of this consistency, we generated a correction factor with 95% prediction intervals for Tono-Pen readings. CONCLUSIONS The Tono-Pen 2 can be used reliably to measure IOP in the normal rat eye.