The effects of seasonal changes on the molecular weight of Nephila clavata spider silk

The effects of seasonal changes on the molecular weight of Nephila clavata spider silk
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季节变化对棒新妇蜘蛛丝分子量的影响

DOI:
10.1038/pj.2015.138
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发表时间:
2016
期刊:
影响因子:
2.8
通讯作者:
Takashi Matsuhira and Hiromi Sakai
Takashi Matsuhira and Hiromi Sakai
中科院分区:
化学3区
文献类型:
--
作者:
Shigeyoshi Osaki;Keizo Yamamoto;Takashi Matsuhira and Hiromi Sakai

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相似文献

研究人员从日本秋天的不同日子收集到的Nephila clavata蜘蛛腹部的主要壶状腺中提取了丝蛋白。用电泳法测定了125只蜘蛛蛛丝蛋白在还原状态下的分子量。结果表明,在秋季季节,蜘蛛丝蛋白的分子量随时间的变化而变化,在交配季节达到峰值约300 kDa。除交配季节外,平均分子量为~ 270 kDa。雌性蜘蛛的分子量如此之高,可能是由于需要构建机械强度强的球形蛛网,这种蛛网由丝和相对较大的蛋白质组成,目的是容纳许多雄性蜘蛛。
Silk proteins were taken from the major ampullate glands found in the abdomens of Nephila clavata spiders that were collected on different days during an autumn season in Japan. The molecular weights of the silk proteins taken from 125 spiders were determined under a reduced state using electrophoresis. It was found that the molecular weight of N. clavata spider silk protein changed depending on the time period during the autumn season and showed a peak value of~ 300 kDa during mating season. However, the molecular weight of N. clavata spider silk protein was, on average,~ 270 kDa except during mating season. Such a peak in the molecular weight for female N. clavata may be ascribed to the necessity for building mechanically strong orb-webs, which consist of silk with relatively large proteins for the purpose of accepting many male spiders.
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发表时间: 2012-04
影响因子: 8.6
作者:
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