An investigation on the cytotoxicity and caspase-mediated apoptotic effect of biologically synthesized silver nanoparticles using Podophyllum hexandrum on human cervical carcinoma cells
An investigation on the cytotoxicity and caspase-mediated apoptotic effect of biologically synthesized silver nanoparticles using Podophyllum hexandrum on human cervical carcinoma cells
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DOI：
10.1016/j.colsurfb.2012.09.042
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发表时间：
2013-02-01
影响因子：
5.8
通讯作者：
Ganapathi, Andy
中科院分区：
文献类型：
--
作者：
Jeyaraj, Murugaraj;Rajesh, Manoharan;Ganapathi, Andy
目前，通过生物实体合成和表征银纳米粒子(AgNPs)在各种生物医学中的应用，特别是癌症的治疗，是非常有趣的。本文介绍了以鬼针叶提取物为原料，通过改变pH值、温度、反应时间、萃取物体积和金属离子浓度等工艺参数进行优化，实现了AgNPs的绿色合成。采用透射电子显微镜、X射线衍射仪和傅立叶变换红外光谱对其进行了表征。结果表明，所合成的纳米粒子为球形，平均尺寸为14 nm。采用四甲基偶氮唑盐比色法、ROS定量、RT-PCR和Western blotting技术分析AgNPs对人宫颈癌细胞的杀伤作用。结果表明，AgNPs可通过DNA损伤和caspase介导的细胞死亡选择性抑制HeLa的细胞机制。这种合成AgNPs和选择性抑制癌细胞的生物程序为有效治疗人类癌症提供了一条替代途径。(C)2012爱思唯尔B.V.保留所有权利。
Now-a-days synthesis and characterization of silver nanoparticles (AgNPs) through biological entity is quite interesting to employ AgNPs for various biomedical applications in general and treatment of cancer in particular. This paper presents the green synthesis of AgNPs using leaf extract of Podophyllum hexandrum Royle and optimized with various parameters such as pH, temperature, reaction time, volume of extract and metal ion concentration for synthesis of AgNPs. TEM, XRD and FTIR were adopted for characterization. The synthesized nanoparticles were found to be spherical shaped with average size of 14 nm. Effects of AgNPs were analyzed against human cervical carcinoma cells by MTT Assay, quantification of ROS, RT-PCR and western blotting techniques. The overall result indicates that AgNPs can selectively inhibit the cellular mechanism of HeLa by DNA damage and caspase mediated cell death. This biological procedure for synthesis of AgNPs and selective inhibition of cancerous cells gives an alternative avenue to treat human cancer effectively. (c) 2012 Elsevier B.V. All rights reserved.