Isolation, characterization and mRNA expression of four cDNAs encoding translation elongation factor 1A from rice (Oryza sativa L.)
Isolation, characterization and mRNA expression of four cDNAs encoding translation elongation factor 1A from rice (Oryza sativa L.)
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DOI：
10.1023/a:1005960721762
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发表时间：
1998-01-01
影响因子：
5.1
通讯作者：
Ejiri, S
中科院分区：
文献类型：
--
作者：
Kidou, S;Ejiri, S
从水稻(Oryza sativa L.)中分离到4个不同的蛋白质合成伸长因子1a(EEF1a)基因克隆。这些基因编码的基因分别是水稻伸长因子1A基因refa1、refa2、refa3和refa4。这些基因编码相同的EEF-1A多肽，并与其他真核生物的EEF-1A有很高的氨基酸同源性。Southern杂交分析表明，这些I-EFA基因中的一些可能在短距离内排列在同一染色体上。对水稻基因组DNA进行的PCR分析表明，refa1和refa4以及refa3和refa2位于水稻基因组的相邻位置。在不同的组织和不同的生长阶段，四种REFA基因的mRNAs积累到几乎相同的水平。悬浮培养细胞中含有最丰富的refA mRNAs。休眠的种子含有少量的四种REFA mRNAs。在种子萌发后，转录产物的积累被高度诱导，即使在成熟植株的老叶片中也保持着相同的表达水平。
Four different cDNA clones encoding protein synthesis elongation factor 1A, eEF1A, were isolated from rice (Oryza sativa L.). The genes encoded by these cDNAs were designated rice elongation factor 1A genes refa1, refa2, refa3 and refa4. The genes encoded identical eEF-1A polypeptides and shared high amino acid identity with eEF1A of other eukaryotes. Southern blot analysis suggested that some of these I-efa genes may be organized in a cluster on the same chromosome within a short distance. PCR analysis of rice genomic DNA showed that refa1 and refa4, and refa3 and refa2 are in neighboring locations on the rice genome. The mRNAs of the four refa genes accumulated to nearly equal levels in a variety of tissues and at different stages of growth. Suspension-cultured cells were the most abundant in refa mRNAs. Dormant seeds contained a small amount of the four refa mRNAs. Transcript accumulation was highly induced after seed germination, and the same expression levels were maintained even in old leaf blades of mature plants.