NINJA connects the co-repressor TOPLESS to jasmonate signalling.
NINJA connects the co-repressor TOPLESS to jasmonate signalling.
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茉莉酰异亮氨酸 (JA-Ile) 是一种植物激素，可调节多种植物防御和发育过程。 JA-Ile 响应基因表达由转录激活因子 MYC2 调节，MYC2 与茉莉酸 ZIM 结构域 (JAZ) 阻遏蛋白发生物理相互作用。当 JA-Ile 感知时，JAZ 蛋白被降解并且 JA-Ile 依赖性基因表达被激活。 JAZ 蛋白抑制基因表达的分子机制仍不清楚。在这里，我们展示了 JAZ 蛋白通过先前未表征的接头蛋白（称为 JAZ 的新型 INteractor (NINJA)）招募 Groucho/Tup1 型共阻遏物 TOPLESS (TPL) 和 TPL 相关蛋白 (TPR)。 NINJA 充当转录抑制因子，其活性由功能性 TPL 结合 EAR 抑制基序介导。因此，NINJA 和 TPL 蛋白均充当茉莉酸反应的负调节因子。我们的结果表明 TPL 蛋白作为通用共阻遏物，通过与特定接头蛋白相互作用影响多种信号传导途径。这一新见解揭示了与胁迫和生长相关的信号级联如何利用常见的分子机制来调节植物中的基因表达。
Jasmonoyl-isoleucine (JA-Ile) is a plant hormone that regulates a broad array of plant defence and developmental processes. JA-Ile-responsive gene expression is regulated by the transcriptional activator MYC2 that interacts physically with the jasmonate ZIM-domain (JAZ) repressor proteins. Upon JA-Ile perception, JAZ proteins are degraded and JA-Ile-dependent gene expression is activated. The molecular mechanisms by which JAZ proteins repress gene expression remain unknown. Here we show that the JAZ proteins recruit the Groucho/Tup1-type co-repressor TOPLESS (TPL) and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel INteractor of JAZ (NINJA). NINJA acts as a transcriptional repressor of which the activity is mediated by a functional TPL-binding EAR repression motif. Accordingly, both NINJA and TPL proteins function as negative regulators of jasmonate responses. Our results point to TPL proteins as general co-repressors that affect multiple signalling pathways through the interaction with specific adaptor proteins. This new insight reveals how stress- and growth-related signalling cascades use common molecular mechanisms to regulate gene expression in plants.
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