Blue-light-induced PIN3 polarization for root negative phototropic response in Arabidopsis.
Blue-light-induced PIN3 polarization for root negative phototropic response in Arabidopsis.
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DOI：
10.1111/tpj.12298
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发表时间：
2013-10
期刊：
影响因子：
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通讯作者：
Kun-Xiao Zhang;Heng-Hao Xu;Ting-Ting Yuan-Ting;Liang Zhang;Ying‐Tang Lu
中科院分区：
文献类型：
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作者：
Kun-Xiao Zhang;Heng-Hao Xu;Ting-Ting Yuan-Ting;Liang Zhang;Ying‐Tang Lu
根系负向光性是植物的一种重要反应。虽然已知蓝光介导这种反应，但根负向光性的细胞和分子机制尚不清楚。本文报道了生长素外排载体PIN- formed (PIN) 3参与生长素不对称分布和根系负向光性。单侧蓝光照射使PIN3偏光到受光照根侧小柱细胞外外侧膜，增加根受光照侧生长素活性，生长素促进生长，使根弯曲远离光源。此外，根的负向光敏反应和蓝光诱导的PIN3极化是由brefeldin a敏感的、gnome依赖的转运途径和p1调节的PINOID (PID)/PROTEIN PHOSPHATASE 2A （PP2A）活性调节的。我们的研究结果表明，蓝光诱导的PIN3极化是根系负向光敏反应中生长素分布不对称的必要条件。
Root negative phototropism is an important response in plants. Although blue light is known to mediate this response, the cellular and molecular mechanisms underlying root negative phototropism remain unclear. Here, we report that the auxin efflux carrier PIN-FORMED (PIN) 3 is involved in asymmetric auxin distribution and root negative phototropism. Unilateral blue-light illumination polarized PIN3 to the outer lateral membrane of columella cells at the illuminated root side, and increased auxin activity at the illuminated side of roots, where auxin promotes growth and causes roots bending away from the light source. Furthermore, root negative phototropic response and blue-light-induced PIN3 polarization were modulated by a brefeldin A-sensitive, GNOM-dependent, trafficking pathway and by phot1-regulated PINOID (PID)/PROTEIN PHOSPHATASE 2A (PP2A) activity. Our results indicate that blue-light-induced PIN3 polarization is needed for asymmetric auxin distribution during root negative phototropic response.