Infrared spectroscopy of normal and abnormal cervical smears: Evaluation by principal component analysis
Infrared spectroscopy of normal and abnormal cervical smears: Evaluation by principal component analysis
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DOI：
10.1006/gyno.1997.4627
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发表时间：
1997-07-01
影响因子：
4.7
通讯作者：
Rigas, B
中科院分区：
文献类型：
--
作者：
Cohenford, MA;Godwin, TA;Rigas, B
恶性和发育不良宫颈刮片的傅立叶变换红外(FT-IR)光谱是异常的，正如我们在有限数量的样本研究中首次描述的那样，其中光谱是通过肉眼检查和峰值强度比来评估的。我们扩大了我们的研究范围，以评估更多的宫颈状况，并通过化学计量学方法(主成分分析[PCA])分析光谱。对436名女性的宫颈样本进行了FT-IR和巴氏试验，其中40/436的光谱无法分析。其余病例如下：正常174例；恶性19例；不典型增生8例；非典型性113例；萎缩性19例；炎症性47例；血涂片12例；细胞减少4例。主成分分析和X(2)检验表明，FT-IR预测恶性的有统计学意义的频率与诊断为恶性的样本相关(P<；0.0001)，以及那些被诊断为“萎缩症”(P<；0.001)、“不典型伴血涂片”(P<；0.05)、“不典型伴萎缩型”(P<；0.05)和“异型增生”(P<；0.05)。根据这些发现，FT-IR诊断宫颈癌的敏感性为79%，特异性为77%，阳性预测值为15%，阴性预测值为98.6%。我们的发现(A)证明了化学计量学方法在研究宫颈FT-LR光谱中的应用；(B)评估了其潜在的诊断作用；(C)表明萎缩和肿瘤样本具有相同的结构特征；以及(D)表明血液可能会干扰这种光谱评估。这些发现为进一步评估FT-IR光谱在宫颈和其他恶性肿瘤中的应用提供了依据。(C)1997年学术出版社。
Fourier-transform infrared (FT-IR) spectra of malignant and dysplastic cervical scrapings were abnormal, as first described in our study of a limited number of samples, where the spectra were evaluated by visual inspection and peak intensity ratios. We have expanded our study to evaluate more cervical conditions, and to analyze the spectra by a chemometric approach (principal component analysis [PCA]). Cervical samples from 436 females were evaluated by FT-IR and Papanicolaou testing; 40/436 spectra were nonanalyzable. The remaining were as follows: normal, 174; malignant, 19; dysplasia, 8; atypia, 113; atrophy, 19; inflammatory, 47; bloody smear, 12; hypocellular, 4. PCA analysis followed by chi(2) test revealed that statistically significant frequencies of being predicted malignant by FT-IR were associated with samples diagnosed as malignant (P < 0.0001), and also those diagnosed as ''atrophy'' (P < 0.001), ''atypical with bloody smear'' (P < 0.05), ''atypical with atrophic pattern'' (P < 0.05), and ''dysplasia'' (P < 0.05). Based on these findings, for the diagnosis of cervical cancer by FT-IR, as defined here, the sensitivity is 79%, the specificity is 77%, the positive predictive value is 15%, and the negative predictive value is 98.6%. Our findings (a) demonstrate the application of a chemometric approach to the study of cervical FT-LR spectra; (b) assess its potential diagnostic role; (c) suggest that atrophic and neoplastic samples share structural features; and (d) suggest that blood may interfere with such spectroscopic evaluation. These findings warrant further evaluation of FT-IR spectroscopy in cervical and other malignancies. (C) 1997 Academic Press.