AIRR Community Guide to Planning and Performing AIRR-Seq Experiments.
AIRR Community Guide to Planning and Performing AIRR-Seq Experiments.
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AIRR 社区规划和执行 AIRR-Seq 实验指南。
DOI：
10.1007/978-1-0716-2115-8_15
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
AIRRCommunity
中科院分区：
文献类型：
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作者：
Eugster,Anne;Bostick,MagnoliaL;Gupta,Nidhi;Mariotti-Ferrandiz,Encarnita;Kraus,Gloria;Meng,Wenzhao;Soto,Cinque;Trück,Johannes;Stervbo,Ulrik;LuningPrak,ElineT;AIRRCommunity
适应性免疫受体库的高通量测序（IG和TR重排的AIRR-seq）的发展为深入分析免疫系统提供了新的前沿。在过去的十年里，协议、实验方法和计算工具出现了爆炸式增长。在本章中，我们讨论了规划一个成功的AIRR-seq实验的主要考虑因素，以及控制和评估实验结果的基本策略。AIRR社区的成员撰写了本版本中的几个章节，其中包括成功进行，分析和共享AIRR-seq项目的分步说明。
The development of high-throughput sequencing of adaptive immune receptor repertoires (AIRR-seq of IG and TR rearrangements) has provided a new frontier for in-depth analysis of the immune system. The last decade has witnessed an explosion in protocols, experimental methodologies, and computational tools. In this chapter, we discuss the major considerations in planning a successful AIRR-seq experiment together with basic strategies for controlling and evaluating the outcome of the experiment. Members of the AIRR Community have authored several chapters in this edition, which cover step-by-step instructions to successfully conduct, analyze, and share an AIRR-seq project.