Differentiation of phagocytic monocytes into lymph node dendritic cells in vivo
Differentiation of phagocytic monocytes into lymph node dendritic cells in vivo
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DOI：
10.1016/s1074-7613(00)80149-1
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发表时间：
1999-12-01
期刊：
影响因子：
32.4
通讯作者：
Muller, WA
中科院分区：
文献类型：
--
作者：
Randolph, GJ;Inaba, K;Muller, WA
我们研究了吞噬皮下注射的荧光微球的炎性单核细胞的分化和运输。正如预期的那样，大多数单核细胞变成微球（+）巨噬细胞，保留在皮下组织中。然而，大约25%的乳胶（+）细胞迁移到引流淋巴结的T细胞区域，在那里它们表达树突状细胞（DC）限制性标记物和高水平的共刺激分子。微球转运细胞与常驻皮肤DC不同，在单核细胞缺陷型骨硬化小鼠中，这种转运减少了85%以上。因此，相当少的炎性单核细胞携带吞噬的颗粒到淋巴结并分化成DC。
We investigated the differentiation and trafficking of inflammatory monocytes that phagocytosed subcutaneously injected fluorescent microspheres. As expected, most of the monocytes became microsphere(+) macrophages, which remained in subcutaneous tissue. However, about 25% of latex(+) cells migrated to the T cell area of draining lymph nodes, where they expressed dendritic cell (DC)-restricted markers and high levels of costimulatory molecules. Microsphere-transporting cells were distinct from resident skin DCs, and this transport was reduced by more than 85% in monocyte-deficient osteopetrotic mice. Thus, a substantial minority of inflammatory monocytes carry phagocytosed particles to lymph nodes and differentiate into DCs.