The Arabidopsis Pleiotropic Drug Resistance Transporters PEN3 and PDR12 Mediate Camalexin Secretion for Resistance to Botrytis cinerea
The Arabidopsis Pleiotropic Drug Resistance Transporters PEN3 and PDR12 Mediate Camalexin Secretion for Resistance to Botrytis cinerea
复制标题
拟南芥多效耐药转运蛋白 PEN3 和 PDR12 介导 Camalexin 分泌以抵抗灰霉病
DOI：
10.1105/tpc.19.00239
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发表时间：
2019-09-01
期刊：
影响因子：
11.6
通讯作者：
Meng, Xiangzong
中科院分区：
文献类型：
--
作者：
He, Yunxia;Xu, Juan;Meng, Xiangzong
植物防御通常取决于病原体接触部位抗菌代谢物的合成和靶向递送。拟南芥 (Arabidopsis thaliana) 中的多效性耐药 (PDR) 转运蛋白 PENETRATION3 (PEN3)/PDR8 与多种真菌病原体的抗性有关。然而，PEN3 转运的用于细胞外防御的抗菌代谢物仍不清楚。在这里，我们报告 PEN3 与另一个 PDR 转运蛋白 (PDR12) 冗余地发挥作用，介导拟南芥中主要植物抗毒素 Camalexin 的分泌。与此一致的是，pen3 pdr12双突变体表现出对坏死性真菌灰葡萄孢的敏感性显着增强以及对外源camalexin的严重超敏性。 PEN3 和 PDR12 在 B. cinerea 感染后被转录激活，它们的表达受到丝裂原激活蛋白激酶 3 (MPK3) 和 MPK6 及其下游 WRKY33 转录因子的调节。进一步的遗传分析表明，PEN3 和 PDR12 不仅通过输出camalexin，而且还输出来自色氨酸代谢的其他未鉴定的代谢物，从而有助于灰霉病抗性，这表明PEN3和PDR12通过运输不同的色氨酸代谢产物，在拟南芥免疫中具有多种功能。
Plant defense often depends on the synthesis and targeted delivery of antimicrobial metabolites at pathogen contact sites. The pleiotropic drug resistance (PDR) transporter PENETRATION3 (PEN3)/PDR8 in Arabidopsis (Arabidopsis thaliana) has been implicated in resistance to a variety of fungal pathogens. However, the antimicrobial metabolite(s) transported by PEN3 for extracellular defense remains unidentified. Here, we report that PEN3 functions redundantly with another PDR transporter (PDR12) to mediate the secretion of camalexin, the major phytoalexin in Arabidopsis. Consistent with this, the pen3 pdr12 double mutants exhibit dramatically enhanced susceptibility to the necrotrophic fungus Botrytis cinerea as well as severe hypersensitivity to exogenous camalexin. PEN3 and PDR12 are transcriptionally activated upon B. cinerea infection, and their expression is regulated by the mitogen-activated protein kinase 3 (MPK3) and MPK6, and their downstream WRKY33 transcription factor. Further genetic analysis indicated that PEN3 and PDR12 contribute to B. cinerea resistance through exporting not only camalexin but also other unidentified metabolite(s) derived from Trp metabolism, suggesting that PEN3 and PDR12 have multiple functions in Arabidopsis immunity via transport of distinct Trp metabolic products.