Estradiol-17β stimulates phosphate uptake and is mitogenic for primary rabbit renal proximal tubule cells
Estradiol-17β stimulates phosphate uptake and is mitogenic for primary rabbit renal proximal tubule cells
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DOI：
10.1159/000065300
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发表时间：
2002-01-01
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影响因子：
--
通讯作者：
Taub, M
中科院分区：
文献类型：
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作者：
Han, HJ;Lee, YH;Taub, M
本文研究了雌二醇-17 β (E-2)对原代兔肾近端小管细胞（ptc）中磷酸（P-i）摄取和DNA合成的直接影响。在本研究中，E-2 （bbb10 (-9) M，超过9天）导致[H-3]胸腺嘧啶掺入和ptc数量增加。抗雌激素的他莫昔芬完全阻止了E-2诱导的[H-3]胸腺嘧啶掺入增加，改善了E-2对生长的刺激作用。E-2 （bbb10 (-9) M，超过5天）也刺激了Pi的吸收，其作用是由于V-max值而不是P-i吸收的K-m值。雌三醇和雌酮对P-i摄取也有显著的刺激作用。黄体酮、他莫昔芬、放线菌素D和环己亚胺阻止e2诱导的P-i摄取刺激。综上所述，生理浓度的雌激素刺激肾近端小管细胞P-i摄取和DNA合成，这些作用是由雌激素受体介导的。版权所有(C) 2002 S. Karger AG，巴塞尔。
The direct effects of estradiol-17beta (E-2) on phosphate (P-i) uptake and on DNA synthesis in the primary rabbit kidney proximal tubule cells (PTCs) have been investigated. In the present study, E-2 (>10(-9) M, over 9 days) causes an increase both in [H-3]thymidine incorporation and the number of PTCs. The anti-estrogen tamoxifen completely prevented the E-2-induced increase in [H-3]thymidine incorporation, and ameliorated the stimulatory effect of E-2 on growth. E-2 (>10(-9) M, over 5 days) also stimulated the Pi uptake and its effect was due to the V-max values but not to the K-m value for P-i uptake. Estriol and estrone also exerted significant stimulatory effects on P-i uptake. Progesterone, tamoxifen, actinomycin D and cycloheximide prevented the E-2-induced stimulation of P-i uptake. In conclusion, estrogens at physiological concentrations stimulate P-i uptake and DNA synthesis in the renal proximal tubule cells, and these effects are estrogen receptor mediated. Copyright (C) 2002 S. Karger AG, Basel.