The depletion of MARVELD1 leads to murine placenta accreta via integrin β4-dependent trophoblast cell invasion
The depletion of MARVELD1 leads to murine placenta accreta via integrin β4-dependent trophoblast cell invasion
复制标题
MARVELD1 的耗竭通过整合素β4 依赖的滋养层细胞入侵导致小鼠胎盘植入。
DOI：
10.1002/jcp.26098
复制
发表时间：
2018-03-01
影响因子：
5.6
通讯作者：
Li, Yu
中科院分区：
文献类型：
--
作者：
Chen, Yue;Zhang, Hui;Li, Yu
胎盘是一个非凡的器官，它是母亲和胎儿之间的界面。滋养层细胞的适当侵袭是成功妊娠所必需的。先前的研究发现粘附分子整合素β4在滋养层细胞侵袭过程中发挥重要作用。在这里，我们发现 MARVELD1 敲除小鼠的总体出生率远低于野生型小鼠（p < 0.001）。在E18.5 MARVELD1敲除小鼠中，我们观察到滋养层细胞的过度侵袭，事实上，怀孕的小鼠具有部分植入性胎盘表型。 HTR8/SVneo 细胞系被用作体外模型来阐明 MARVELD1 介导的滋养层侵袭的潜在机制。我们检测到 MARVELD1 下调后整合素 β 4 的表达减少，并且滋养层细胞在体内和体外的迁移和侵袭能力增强。整合素β4救援试验也支持了这一结果。总之，本研究发现MARVELD1在胎盘发育过程中通过调节整合素β4的表达来介导滋养层细胞的侵袭。
The placenta is a remarkable organ, it serves as the interface between the mother and the fetus. Proper invasion of trophoblast cells is required for a successful pregnancy. Previous studies have found that the adhesion molecule integrin beta 4 plays important roles during trophoblast cell invasion. Here, we found that the overall birth rate of the MARVELD1 knockout mouse is much lower than that of the wild-type mouse (p < 0.001). In E18.5 MARVELD1 knockout mice, we observed an over-invasion of trophoblast cells, and indeed, the pregnant mice had a partial placenta accreta phenotype. The HTR8/SVneo cell line was used as an in vitro model to elucidate the underlying mechanisms of MARVELD1-mediated trophoblast invasion. We detected a diminished expression of integrin beta 4 upon the downregulation of MARVELD1 and enhanced migrate and invasive abilities of trophoblast cells both in vivo and in vitro. The integrin beta 4 rescue assay also supported the results. In conclusion, this study found that MARVELD1 mediated the invasion of trophoblast cells via regulating the expression of integrin beta 4 during placenta development.