Physicochemical stability and inactivation of human and simian rotaviruses
Physicochemical stability and inactivation of human and simian rotaviruses
复制标题
人和猿轮状病毒的理化稳定性和灭活
DOI：
10.1128/aem.53.4.727-730.1987
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发表时间：
1987
影响因子：
4.4
通讯作者：
I. Gust
中科院分区：
文献类型：
--
作者：
Z. Meng;C. Birch;R. Heath;I. Gust
用荧光聚焦法比较了不同物理和化学处理对人血清1型轮状病毒和猴源病毒11（SA 11）稳定性的影响。在室温下储存14天、4 ℃下储存22天和-20 ℃下储存32天;冻干;并在pH 3至11下处理后，两种菌株的感染性均得以保留。两种病毒在pH 12下灭活，人病毒在pH 2下也是如此，尽管该pH仅导致SA11的部分灭活。人病毒对乙醚和氯仿的作用似乎也比SA 11更敏感。两种病毒的感染性后，紫外线照射15分钟，并与8%的甲醛5分钟，70%（体积/体积）乙醇30分钟，2%的来苏，2%的苯酚，和1%H2O2处理1小时后失去。
The effects of various physical and chemical treatments on the stability of a human serotype 1 rotavirus and simian agent 11 (SA11) were compared by using a fluorescence focus assay. The infectivity of both strains was retained after storage at room temperature for 14 days, 4 degree C for 22 days, and -20 degree C for 32 days; lyophilization; and treatment at pH 3 to 11. Both viruses were inactivated at pH 12, as was the human virus at pH 2, although this pH resulted in only partial inactivation of SA11. The human virus also appeared to be more sensitive than SA11 to the action of ether and chloroform. The infectivity of both viruses was lost after UV irradiation for 15 min and after treatment with 8% formaldehyde for 5 min, 70% (vol/vol) ethanol for 30 min, and 2% lysol, 2% phenol, and 1% H2O2 for 1 h each.