Regarding flagellar expression in clinical isolates of non-typeable Haemophilus influenzae.
Regarding flagellar expression in clinical isolates of non-typeable Haemophilus influenzae.
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关于不可分型流感嗜血杆菌临床分离株中鞭毛的表达。
DOI：
10.1099/jmm.0.000632
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发表时间：
2017
影响因子：
3
通讯作者：
Bossé JT
中科院分区：
文献类型：
--
作者：
Bossé JT
流感嗜血杆菌（HI）历来被认为是一种非运动性、非鞭毛虫细菌。因此，Carabarin-Lima et al. [1]描述了许多HI菌株中的“群集运动性”，并且在菌株BUAP 96中存在两个鞭毛基因。其中一个被报道为能动的菌株是Rd KW 20（Rd），这是第一个对其全基因组测序的细菌[2]，其不具有任何已知的鞭毛基因。作者使用基于已知肠基因的“简并”引物扩增来自BUAP 96的1490（fliC）和700（flgH）bp的弱条带，加上来自Rd的约850 bp（flgH）的未注释条带（图2）。我们不能预测，通过虚拟PCR，使用Rd全基因组序列（登录号：L42023）和所述引物的任何扩增子。据报道，BUAP 96 fliC的5′端和3′端测序与已知的肠鞭毛蛋白基因以及胸膜肺炎放线杆菌（APP）fliC样基因具有“高度同源性”[3]，但尚未得到证实。然而，没有BUAP 96 fliC（或flgH）的序列数据被呈现或公开可用。
Haemophilus influenzae (HI) has historically been considered to be a non-motile, non-flagellate bacterium. It was, therefore, of considerable interest that Carabarin-Lima et al.[1] described ‘swarming motility’in numerous HI strains, and the presence of two flagella genes in strain BUAP96. One of the strains reported as motile was Rd KW20 (Rd), the first bacterium to have its whole genome sequenced [2], which does not possess any known flagella genes.The authors used ‘degenerate’primers based on known enteric genes to amplify weak bands of 1490 (fliC) and 700 (flgH) bp from BUAP96, plus an uncommented on circa 850bp (flgH) band from Rd (Fig. 2). We were unable to predict, by virtual PCR, any amplicon using the Rd wholegenome sequence (accession number: L42023) and the primers stated. The sequenced 5′ and 3′ ends of BUAP96 fliC were reported to share ‘high homology’with known enteric flagellin genes, as well as an Actinobacillus pleuropneumoniae (APP) fliC-like gene [3], which has not been substantiated. However, no sequence data for BUAP96 fliC (or flgH) were presented, or made publicly available.