Expression, enzyme activity, and subcellular localization of mammalian target of rapamycin in insulin-responsive cells.
Expression, enzyme activity, and subcellular localization of mammalian target of rapamycin in insulin-responsive cells.
复制标题
胰岛素反应细胞中雷帕霉素哺乳动物靶标的表达、酶活性和亚细胞定位。
DOI：
10.1006/bbrc.1997.7878
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Shepherd,PR
中科院分区：
文献类型：
--
作者：
Withers,DJ;Ouwens,DM;Nave,BT;vanderZon,GC;Alarcon,CM;Cardenas,ME;Heitman,J;Maassen,JA;Shepherd,PR
The role of the mammalian target of rapamycin (mTOR) was investigated in insulin responsive cell lines. mTOR was expressed at high levels in insulin responsive cell types and in 3T3-L1 cells mTOR expression levels increased dramatically as cells differentiated from fibroblasts into insulin responsive adipocytes. mTOR localized to membrane fractions in all cells tested and in 3T3-L1 adipocytes mTOR was specifically localized to microsomal membranes. Protein kinase activity directed towards mTOR was tightly associated with mTOR immunoprecipitates and this kinase activity was inhibited by FKBP12-rapamycin indicating it was due to an autokinase activity present in mTOR. The mTOR autokinase and the protein kinase activity of the p110α isoform of PI 3-kinase shared several notable similarities; (a) both were maximally active in the presence of Mn2+but also showed significant activity in the presence of Mg2+(b) neither were inhibited by the presence of non-ionic detergent and (c) both were inhibited by wortmannin and LY294002, known inhibitors of the PI 3-kinase lipid kinase activity. These data taken together indicate the autokinase activity lay in the PI 3-kinase homology domain. In summary mTOR is a membrane anchored protein kinase that is active in conditions encountered in vivo and the fact it is highly expressed in insulin responsive cell types is consistent with a role in insulin signalling.
正常和胰岛素抵抗小鼠骨骼肌中丝裂原激活蛋白激酶、p90rsk 和 p70 S6 激酶的胰岛素刺激
影响因子：
4.8
作者：
P. Chang;Y. Le Marchand;L. Cheatham;D. Moller
通讯作者：
D. Moller
通过细胞体积的变化调节大鼠骨骼肌中的糖原合成。
DOI：
10.1113/jphysiol.1996.sp021594
发表时间：
1996
期刊：
The Journal of Physiology
影响因子：
--
作者：
S. Low;M. Rennie;P. Taylor
通讯作者：
P. Taylor