Cloning of a novel protease required for the molting of Locusta migratoria manilensis
Cloning of a novel protease required for the molting of Locusta migratoria manilensis
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DOI：
10.1111/j.1440-169x.2007.00957.x
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发表时间：
2007-08
期刊：
影响因子：
4.6
通讯作者：
Zhang Wei;Youping Yin;Basheng Zhang;Zhong-kang Wang;Guoxiong Peng;Yueqing Cao;Yuxian Xia
中科院分区：
文献类型：
--
作者：
Zhang Wei;Youping Yin;Basheng Zhang;Zhong-kang Wang;Guoxiong Peng;Yueqing Cao;Yuxian Xia
蜕皮是昆虫生命周期中幼虫阶段之间的进展所必需的。昆虫蜕皮的实质是新角质层的形成和旧角质层的脱落。旧角质层的降解和回收是由蜕皮液中的酶引起的，这些酶填充了新旧角质层之间的空间。在这里，我们描述了一个新的蛋白酶基因从东亚飞蝗，命名为Lm-TSP的克隆。cDNA及其推导的蛋白质序列已在GenBank中登录（登录号分别为EF 081255和ABN 13876）。序列分析表明Lm-TSP属于胰蛋白酶样丝氨酸蛋白酶家族。我们通过RNA干扰（RNAi）表明，Lm-TSP的沉默导致蜕皮液的蛋白酶和角质层降解活性急剧降低，这导致从四龄幼虫（L4）到五龄幼虫（L5）以及L5和成虫阶段之间的蜕皮缺陷。这些观察结果表明，Lm-TSP在L.东亚飞蝗蜕皮
Molting is required for progression between larval stages in the life cycle of an insect. The essence of insect molting is the laying down of new cuticle followed by shedding of the old cuticle. Degradation and recycling of old cuticle are brought about by enzymes present in the molting fluid, which fills the space between the old and new cuticle. Here, we describe the cloning of a novel protease gene from Locusta migratoria manilensis, designated as Lm‐TSP. The cDNA and its deduced protein sequences were deposited in GenBank (accession numbers EF081255 and ABN13876, respectively). Sequence analysis indicated that Lm‐TSP belongs to the trypsin‐like serine protease family. We show, by RNA interference (RNAi), that silencing of Lm‐TSP leads to dramatic reductions in protease and cuticle‐degrading activity of a molting fluid, which leads to molting defects from fourth‐instar larvae (L4) to fifth‐instar larvae (L5), and between L5 and adult stages. These observations suggest that Lm‐TSP plays a critical role in L. migratoria manilensis ecdysis.