Abnormal platelet and lymphocyte calcium handling in prehypertensive rats.
Abnormal platelet and lymphocyte calcium handling in prehypertensive rats.
复制标题
高血压前期大鼠血小板和淋巴细胞钙处理异常。
DOI：
10.1161/01.hyp.18.1.111
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
McCarron,DA
中科院分区：
文献类型：
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作者：
Oshima,T;Young,EW;McCarron,DA
我们报道了12-14周龄自发性高血压大鼠(SHR)的基础和刺激细胞内游离钙离子浓度[(Ca~(2+)]_i)较正常血压的Wistar-京都(WKY)大鼠升高。为了确定细胞钙代谢改变是否先于显性高血压的发生，我们测定了4周龄高血压前期SHR和WKY大鼠的血小板和胸腺淋巴细胞在静息和刺激条件下的[Ca~(2+)]i。两组血压相似(SHR 95+/-8对WKY大鼠92+/-7 mm Hg)。自发性高血压大鼠的基础[Ca~(2+)]i高于WKY大鼠(63.4+/-3.9nM比54.8+/-3.1nM，p<0.003)。此外，无论有无细胞外钙，SHR大鼠对凝血酶的[Ca~(2+)]i反应均大于WKY大鼠。淋巴细胞基础[Ca~(2+)]_i无明显变化，但在有钙和无钙条件下，SHR的[Ca~(2+)]_i峰值均高于WKY大鼠。这些结果表明，激动剂刺激的钙内流和细胞内钙排出在4周龄自发性高血压大鼠的血小板和淋巴细胞中均有增加。我们得出结论，在SHR中，两种不同细胞类型的钙代谢异常先于明显高血压的发生。
We have reported that the basal and stimulated cytosolic free calcium concentrations [( Ca2+]i) are elevated in platelets isolated from 12-14-week-old spontaneously hypertensive rats (SHR) as compared with normotensive Wistar-Kyoto (WKY) rats. To determine whether altered cell calcium metabolism precedes the development of overt hypertension, we measured [Ca2+]i under resting and stimulated conditions in blood platelets and thymic lymphocytes isolated from 4-week-old prehypertensive SHR and WKY rats. Blood pressure was similar in both groups (SHR 95 +/- 8 versus WKY rats 92 +/- 7 mm Hg). Basal [Ca2+]i in platelets was higher in SHR than WKY rats (63.4 +/- 3.9 versus 54.8 +/- 3.1 nM, p less than 0.003). Also the [Ca2+]i response to thrombin was greater in SHR than WKY rats in both the presence and absence of extracellular calcium. For lymphocytes, although no difference was detected in basal [Ca2+]i, the concanavalin A-induced peak [Ca2+]i was higher for SHR than WKY rats in both calcium-containing and calcium-free media. These results suggest that agonist-stimulated calcium influx and calcium discharge from intracellular stores are enhanced in both platelets and lymphocytes of 4-week-old SHR. We conclude that abnormalities in calcium metabolism in two different cell types precede the development of overt hypertension in the SHR.