The role of catechin and epicatechin in chemical defense against damping‐off fungi of current‐year Fagus crenata seedlings in natural forest
The role of catechin and epicatechin in chemical defense against damping‐off fungi of current‐year Fagus crenata seedlings in natural forest
复制标题
儿茶素和表儿茶素对天然林当年生水青冈苗木猝倒真菌的化学防御作用
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Y. Ichihara
中科院分区：
文献类型：
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作者：
K. Yamaji;Y. Ichihara
总结
Summary
Current-year Fagus crenata seedlings survive damping-off in the forest-edge plot compared to those in the closed-stand plot. Greater illumination found in the forest-edge plot is likely to influence the defense of seedlings against pathogenic attack, by increasing the amount of phenolic compounds and forming periderm. This study aimed to clarify the chemical defense system by (i) isolation of antifungal compounds from seedling hypocotyls in the forest-edge plot, (ii) quantification of antifungal compounds in hypocotyls in the forest-edge and closed-stand plots, (iii) estimation of their antifungal activities against Cylindrocarpon sp. and Colletotrichum dematium and (iv) clarification of localization of phenolics in the hypocotyl tissues. Catechin and epicatechin were isolated as antifungal compounds showing inhibition of microconidia germination of Cylindrocarpon sp. and mycelial growth of Cylindrocarpon sp. and C. dematium. In the beginning of July when damping-off was the most severe in the closed-stand plot, the total amount of catechin and epicatechin in the forest-edge seedlings was approximately three times greater than that in the closed-stand seedlings. Flavan-3-ols, including catechin and epicatechin, were distributed mainly in the epidermal tissue on June 1 and in cortical tissues on June 15 and July 6, all from plants harvested from the forest-edge plot. We conclude that F. crenata could survive damping-off through chemical defense via catechin and epicatechin production and its distribution in seedling hypocotyls.