Spinal Astrocytic Thrombospondin-4 Induced by Excitatory Neuronal Signaling Mediates Pain After Facet Capsule Injury.
Spinal Astrocytic Thrombospondin-4 Induced by Excitatory Neuronal Signaling Mediates Pain After Facet Capsule Injury.
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DOI：
10.1007/s10439-016-1639-x
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发表时间：
2016-11
影响因子：
3.8
通讯作者：
Winkelstein, Beth A.
中科院分区：
文献类型：
--
作者：
Crosby, Nathan D.;Winkelstein, Beth A.
Thrombospondin-4 (TSP4) is a synaptogenic molecule that is upregulated in the spinal cord after painful facet joint injury and may contribute to spinal hyperexcitability. However, the mechanisms leading to increased spinal TSP4 are unclear. Because primary afferent activity is critical in the development of spinal hyperexcitability after facet joint injury, this study evaluated the role of afferent firing in the increase of spinal TSP4 and excitatory synapses. Intra-articular bupivacaine was administered immediately or 4 days after painful facet joint injury in male Holtzman rats, and TSP4 and excitatory synapses were quantified in the spinal cord at day 7. Immediate, but not delayed bupivacaine treatment, prevents the injury-induced increase in TSP4 and excitatory synapses in the dorsal horn (p<0.0001). Preliminary in vitro experiments suggest that the excitatory signaling molecules ATP and glutamate may stimulate astrocytic TSP4 expression (p≤0.04). Collectively, these results suggest that afferent activity early after facet joint injury is critical for the induction of spinal TSP4. This study advances the understanding of the timing and role of afferent activity in TSP4 expression after injury, which is critical for the therapeutic targeting of TSP4 to treat persistent pain conditions.
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DOI：
10.1073/pnas.81.21.6890
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FYFFE, REW;PERL, ER
通讯作者：
PERL, ER
影响因子：
7.4
作者：
Crosby ND;Gilliland TM;Winkelstein BA
通讯作者：
Winkelstein BA
DOI：
10.3791/2270
发表时间：
2010-11-16
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Ippolito, Dominic M;Eroglu, Cagla
通讯作者：
Eroglu, Cagla
影响因子：
2.8
作者：
Kallakuri, Srinivasu;Singh, Anita;Cavanaugh, John M.
通讯作者：
Cavanaugh, John M.
影响因子：
3.3
作者：
Matsuka, Yoshizo;Ono, Takeshi;Spigelman, Igor
通讯作者：
Spigelman, Igor