Green synthesis of silver nanoparticles with bagasse for colorimetric detection of cysteine in serum samples
Green synthesis of silver nanoparticles with bagasse for colorimetric detection of cysteine in serum samples
复制标题
甘蔗渣绿色合成银纳米颗粒用于血清样品中半胱氨酸的比色检测
DOI：
10.1016/j.jallcom.2016.05.348
复制
发表时间：
2016-11-25
影响因子：
6.2
通讯作者：
Sun, Runcang
中科院分区：
文献类型：
--
作者：
Shen, Zuguang;Han, Guocheng;Sun, Runcang
报道了一种以甘蔗渣为还原剂和稳定剂制备银纳米粒子（AgNPs）的绿色方法，用于快速检测L-半胱氨酸（Cys）。通过改变反应物配比、反应温度和反应时间，得到了合成AgNPs的最佳条件。所得银纳米粒子直径为18-28 nm，呈球形，表面包覆有甘蔗渣提取物（BAE），离心后在水中分散良好。BAE/AgNPs复合材料中Ag的含量高达45.4%。探讨了银纳米粒子的可能形成机理。重要的是，BAE/AgNPs复合物显示出Cys的高灵敏度检测，检测限（LOD）为35 nM，并且在19种氨基酸中选择性检测Cys。将BAE/AgNPs复合物应用于人血清中Cys的检测，误差小于5%。因此，本工作提供了一种快速检测半胱氨酸的绿色银纳米粒子的方法，为纳米金属的回收利用提供了方向。(C)2016爱思唯尔B. V.保留所有权利。
This work reported a green method of synthesizing silver nanoparticles (AgNPs) with bagasse acting as reducing and stabilizing agents to rapidly detect L-Cysteine (Cys). The optimal condition of synthesizing AgNPs was obtained by varying the reactant ratio, temperature, and reaction time. The obtained AgNPs with diameters of 18-28 nm were almost spherical and capped by bagasse extract (BAE), and even they were redispersed well in water after centrifugation. The content of Ag in BAE/AgNPs composite was high up to 45.4%. The possible forming mechanism of AgNPs was investigated. Importantly, the BAE/AgNPs composite showed highly sensitive detection of Cys with a limit of detection (LOD) of 35 nM and selective detection of Cys among 19 amino acids. Besides, the BAE/AgNPs composite was applied in Cys detection in human serum with the error less than 5%. Thus, this work provided a rapid method for Cys detection via green-synthesized AgNPs, and the synthesis method provided a direction for recycle of nanometal. (C) 2016 Elsevier B.V. All rights reserved.