THE BASIS FOR COLORED SILVER PROTEIN COMPLEX-FORMATION IN STAINED POLYACRYLAMIDE GELS
THE BASIS FOR COLORED SILVER PROTEIN COMPLEX-FORMATION IN STAINED POLYACRYLAMIDE GELS
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DOI：
10.1016/0003-2697(84)90047-2
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发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
BROWN, LR
中科院分区：
文献类型：
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作者：
NIELSEN, BL;BROWN, LR
使用Merril等人改良的银染法对十二烷基硫酸钠 - 聚丙烯酰胺凝胶中的多肽进行染色，蛋白质条带可重复染成不同深浅的蓝色、黄色、红色和灰色。该过程对温度有高度依赖性，在42℃时显色最佳。该过程可在2小时内完成。显色是由于银离子与带电荷的氨基酸侧链形成复合物。如果已知氨基酸序列，则可以预测银 - 蛋白质复合物的颜色，尽管文中也讨论了一些例外情况。这为通过凝胶电泳表征蛋白质提供了另一个维度。
Using a modified Ag stain of Merril et al. for staining polypeptides in sodium dodecyl sulfate-polyacrylamide gels, protein bands reproducibly stain different shades of blue, yellow, red and gray. The procedure is highly temperature dependent, with optimal color formation at 42.degree. C. The procedure may be completed within 2 h. Color formation is due to Ag ion complexes with charged amino acid side chains. The color of the Ag-protein complex can be predicted if the amino acid sequence is known, although some exceptions are discussed. This provides another dimension to the characterization of proteins by gel electrophoresis.