PARTIAL PANCREATECTOMY IN THE RAT AND SUBSEQUENT DEFECT IN GLUCOSE-INDUCED INSULIN RELEASE
PARTIAL PANCREATECTOMY IN THE RAT AND SUBSEQUENT DEFECT IN GLUCOSE-INDUCED INSULIN RELEASE
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DOI：
10.1172/jci110910
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发表时间：
1983-01-01
影响因子：
15.9
通讯作者：
WEIR, GC
中科院分区：
文献类型：
--
作者：
BONNERWEIR, S;TRENT, DF;WEIR, GC
为了定义已知的β减少的结果，细胞团，进行90%的部分胰腺切除术。术后6周，在进食状态下维持中度高血糖，但与假胰腺切除对照组相比，体重或血浆胰岛素浓度无差异。手术（8-10周）后，残余物的再生很明显，残余物重量为26%，β-细胞质量为42%，非β-细胞质量是对照全胰腺所发现值的47%。胰岛素、胰高血糖素和生长抑素的残余含量也有相当的增加。术后6-7周，经餐后激发、腹膜内和静脉内葡萄糖耐量试验和静脉内精氨酸激发后，胰岛素对葡萄糖的反应减弱或消失，但餐后或精氨酸后的反应完整。同样，当胰腺残体在体外灌注，胰岛素释放后的挑战与300毫克/分升葡萄糖显着减少，而完整的反应，10毫米精氨酸保留。降低的β的慢性刺激-细胞质量可导致葡萄糖诱导的胰岛素分泌的选择性丧失。
To define the consequences of a known reduction of .beta.-cell mass in rats, 90% partial pancreatectomies were performed. For the 6 wk following surgery moderate hyperglycemia was maintained in the fed state but there were no differences in body wt or plasma insulin concentrations compared with sham-pancreatectomized controls. Following surgery (8-10 wk) regeneration of the remnant was evident with remnant weight being 26%, .beta.-cell mass being 42% and non-.beta.-cell mass being 47% of values found for control whole pancreas. There were comparable increases in the remnant content of insulin, glucagon and somatostatin. Following meal challenges, i.p. and i.v. glucose tolerance tests and i.v. arginine challenge given 6-7 wk after surgery, the insulin responses to glucose were blunted or absent but the responses following the meals or arginine were intact. Similarly, when the pancreatic remnant was perfused in vitro, insulin release after challenge with 300 mg/dl glucose was markedly reduced whereas intact responsiveness to 10 mM arginine was retained. The chronic stimulation of a reduced .beta.-cell mass can lead to a selective loss of glucose-induced insulin secretion.