Condensation Pattern (CP) Analysis of Plant Chromosomes by an Improved Chromosome Image Analysing System, CHIAS III
Condensation Pattern (CP) Analysis of Plant Chromosomes by an Improved Chromosome Image Analysing System, CHIAS III
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DOI：
10.1023/a:1009252429277
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发表时间：
1998-09
影响因子：
2.6
通讯作者：
S. Kato;Kiichi Fukui
中科院分区：
文献类型：
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作者：
S. Kato;Kiichi Fukui
第三代染色体图像分析系统（CHIAS III）是使用普通个人电脑和公共领域成像软件NIH image开发的。对甘蔗有丝分裂前期小染色体上出现的不均匀凝聚模式（CP）进行了分析，并利用CHIASⅲ建立了定量染色体图谱。分析包括三个主要步骤：(1)原始染色体图像的处理和CP的测量或CP的数值数据；(2)计算同源染色体的平均CP；(3)基于平均染色体数目的特征图的发展。利用第一代和第二代染色体图象分析系统对染色体数目进行了定量分析。使用CHIAS III获得的数据与先前开发的染色体图像分析系统获得的数据一致。
The third-generation chromosome image analysing system (CHIAS III) was developed by using an ordinary personal computer with public domain imaging software, the NIH Image. An uneven condensation pattern (CP) appearing on small sugarcane chromosomes at the mitotic prometaphase stage was analysed and a quantitative chromosome map or idiogram was developed by using CHIAS III. The analysis consists of three major steps as follows:(1) manipulation of original chromosome images and measurement of CP or numerical data of the CP;(2) calculation of an averaged CP for homologous chromosomes; and (3) development of an idiogram based on the averaged CP. The CP has been analysed quantitatively by using the first-and the second-generation chromosome image analysing systems with hard-and software specific to the systems. The data obtained using CHIAS III are consistent with those obtained with the chromosome image analysing systems previously developed.