N-cadherin is required for the polarized cell behaviors that drive neurulation in the zebrafish
N-cadherin is required for the polarized cell behaviors that drive neurulation in the zebrafish
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DOI：
10.1242/dev.02560
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发表时间：
2006-10-01
期刊：
影响因子：
4.6
通讯作者：
Brewster, Rachel
中科院分区：
文献类型：
--
作者：
Hong, Elim;Brewster, Rachel
通过对细胞行为的直接分析，我们探讨了斑马鱼前神经管形态发生的机制以及细胞粘附分子N-cadherin （N-cad）在这一过程中的作用。我们证明，尽管两栖动物和硬鱼动物之间的神经形成模式在形态学水平上有所不同，但潜在的细胞机制是保守的。与之前的报道相反，斑马鱼神经板是一个多层结构，由深层和表层细胞组成，它们在径向嵌入时向中间汇聚，形成一个单层神经管。单个细胞行为的延时记录显示，细胞沿中外侧轴极化，并表现出突出的活动。在N-cad突变体中，收敛和嵌入都被阻断。此外，尽管n -镉耗尽的细胞在形成突起的能力上没有缺陷，但它们无法稳定地维持它们。综上所述，这些研究揭示了硬骨鱼神经管形态发生的关键细胞机制，并揭示了钙粘蛋白在促进细胞极化行为中的作用，这种极化行为是细胞重排和脊椎动物胚胎形成的基础。
Through the direct analysis of cell behaviors, we address the mechanisms underlying anterior neural tube morphogenesis in the zebrafish and the role of the cell adhesion molecule N-cadherin (N-cad) in this process. We demonstrate that although the mode of neurulation differs at the morphological level between amphibians and teleosts, the underlying cellular mechanisms are conserved. Contrary to previous reports, the zebrafish neural plate is a multi-layered structure, composed of deep and superficial cells that converge medially while undergoing radial intercalation, to form a single cell-layered neural tube. Time-lapse recording of individual cell behaviors reveals that cells are polarized along the mediolateral axis and exhibit protrusive activity. In N-cad mutants, both convergence and intercalation are blocked. Moreover, although N-cad-depleted cells are not defective in their ability to form protrusions, they are unable to maintain them stably. Taken together, these studies uncover key cellular mechanisms underlying neural tube morphogenesis in teleosts, and reveal a role for cadherins in promoting the polarized cell behaviors that underlie cellular rearrangements and shape the vertebrate embryo.