Adenovirus-mediated gene transfer in olfactory epithelium and olfactory bulb: A long-term study
Adenovirus-mediated gene transfer in olfactory epithelium and olfactory bulb: A long-term study
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DOI：
10.1177/000348940511400808
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发表时间：
2005-08-01
影响因子：
1.4
通讯作者：
Terashima, T
中科院分区：
文献类型：
--
作者：
Doi, K;Nibu, K;Terashima, T
目的：研究腺病毒载体在嗅觉通路中介导的时空基因表达。方法：将编码大肠埃希菌p -半乳糖苷酶的复制缺陷腺病毒载体AxCALacZ经鼻灌入小鼠嗅上皮。结果：LacZ基因产物β -半乳糖苷酶不仅在嗅受体神经元及其轴突中表达，而且在嗅球中也有表达。在感染后第2天（PID）观察到第一个顺行标记的证据。在PID 3时，β -半乳糖苷酶在嗅球内嗅神经轴突及其末端肾小球中强烈表达。β -半乳糖苷酶的表达持续到PID 90，并且在PID 30时标记的神经元数量显著减少。结论：这些结果提示腺病毒介导的基因转移可能对嗅神经元和嗅球产生长期影响。
Objectives: We sought to study the spatiotemporal gene expression mediated by adenoviral vector in the olfactory pathways.Methods: The replication-defective adenoviral vector AxCALacZ, which encodes the enzyme Escherichia coli P-galactosidase, was applied to mouse olfactory epithelium by intranasal instillation.Results: The LacZ gene product, beta-galactosidase, was expressed not only in the olfactory receptor neurons and their axons, but also in the olfactory bulbs. The first evidence of anterograde labeling was observed at postinfection day (PID) 2. At PID 3, beta-galactosidase was strongly expressed in olfactory nerve axons, as well as their terminal glomeruli, in the olfactory bulbs. beta-Galactosidase expression persisted up to PID 90, and there was a significant decrease in the number of labeled neurons at PID 30.Conclusions: These results suggest possible long-term effects of adenovirus-mediated gene transfer on the olfactory neurons, as well as the olfactory bulbs.