Low expression of an endopolygalacturonase gene in apple fruit with long-term storage potential

Low expression of an endopolygalacturonase gene in apple fruit with long-term storage potential
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DOI:
10.1016/j.postharvbio.2005.10.005
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发表时间:
2006-02-01
影响因子:
7
通讯作者:
Harada, T
Harada, T
中科院分区:
农林科学1区
文献类型:
--
作者:
Wakasa, Y;Kudo, H;Harada, T

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不同苹果品种成熟过程中果肉软化程度差异很大。世界上最受欢迎的品种富士。即使在室温下储存一个月,也没有发现硬度的损失,而'金冠'在收获后不久就开始软化。为了阐明不同品种之间的软化模式的差异,我们分析了4个成熟相关基因(MdACS1,MdACO1,MdPG1和MdExp3)在14个品种,显示无论是'富士'或'金冠'的硬度损失模式。所有保持坚定的品种显示非常弱或瞬时表达的MdPG 1,无论乙烯生产率和其他基因的转录水平。这些结果表明,在成熟过程中的软化行为可能取决于MdPG 1的表达模式。(c)2005 Elsevier B.V.保留所有权利。
Flesh softening during ripening differs greatly among apple cultivars. 'Fuji', the world's most popular cultivar. shows no detectable loss of firmness even in storage at room temperature for a month, whereas 'Golden Delicious' starts softening soon after harvest. To elucidate how softening patterns differ among cultivars, we analyzed four ripening-related genes (MdACS1, MdACO1, MdPG1 and MdExp3) in 14 cultivars, which display the firmness loss pattern of either 'Fuji' or 'Golden Delicious'. All cultivars that remain firm revealed very weak or transient expression of MdPG1, irrespective of ethylene production rate and transcription levels of other genes. These results suggest that softening behavior during ripening may depend on the expression pattern of MdPG1. (c) 2005 Elsevier B.V. All rights reserved.